Hi everyone - I'm beginning my first trials with the iDISCO+ clearing protocol, and I want to make the buffers in stock quantities in advance, but I'm concerned about the viability with the detergents.
Buffer 1: 2% TritonX-100 in 10X PBS
Buffer 2: glycine and DMSO in buffer 1
Buffer 3: Tween-20 and heparin in 10X PBS
What would be the best way to store these long term?
Thanks in advance for any advice!
This is my opinion:
Buffer 1: I will store for longer term at 4C. Prepare and filter through 20um filter and preferably store in smaller bottles, I may store for months
Buffer 2. I will prepare fresh, keep at 4C and will use in a couple of days
Buffer 3. I will prepare fresh and use the same day and will not store because it has heparin
You may wait for other people's feedback
Good luck
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