Stepwise and overall yield of protein?

More Sadia Kanwal's questions See All

Graphpad prism software full version?

Im looking for Graphpad prism full version. can anyone help me with free download.I searched online but i got the trail version.Please help me to get the full version. Many thanks Best Regards...

03 April 2018 4,492 12 View

Is there any difference between shannon weiner index and simpson index?

What is the main difference between Shannon-Wiener Index and Simpson Index.The definition for both the indices is pretty similar. secondly which index is more appropriate for gut microbial...

02 March 2018 1,778 2 View

Dgge sequence analysis using SNAPGENE?

I got my sequencing results of DGGE(bands) .I'm using SNAPGENE software to retrieve sequences and BLAST in NCBI.I'm not sure if its the correct way to identify bacterial strains.secondly what's...

01 February 2018 8,731 5 View

LPS assay to determine endotoxemia in mice model?

If we want to assess gut permeability, which is the ideal assay to determine Endotoxemia? Does site of blood collection influence the assay?please suggest . Thanks in advance

31 December 2017 2,264 0 View

Skimmed milk or BSA blocking?

Which blocking agent gives more clearer result for Internal control antibody like beta actin ?

31 December 2017 8,520 0 View

Is it necessary to isolate and amplify clones before Sanger sequencing?

Does Sanger sequencing gives clear reads for PCR amplified product followed by purification.can we get precise information about the nucleotide sequencing? Suggestions Please :) Best Regards

10 November 2017 7,534 8 View

Analysis of Intestinal Permeability in mice through FITC-dextran method?

Does FITC-dextran assay has Toxic effect in mice if administered orally before decapitation? does it will effect other tissues and organs? or is there any alternative method(in vitro assay) to...

10 November 2017 8,319 7 View

Physico chemical characterization of Polysaccharide?

What are the critical physico-chemical characterization that should be considered for a novel polysaccharide isolated from mushroom? Thanks

10 November 2017 8,919 3 View

Structure of Polysaccharide?

I want to show structure of different polysaccharides in my manuscript, structures i have obtained by searching on google. My question is how i can cite the structure searched in this way? or i...

10 November 2017 9,598 6 View

How to get the best DGGE gel image?

How I can eliminate smiley(curve bands) on the edge wells in dgge (usually first and last well). Is there any way that I can get straight and clear bands.

10 November 2017 9,570 1 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Are protein samples in SDS compatible with ELISA?

I have used an AllPrep DNA/RNA/Protein Mini Kit QIAGEN kit to extract RNA and protein from my samples. At the end of the protein purification, I resuspend my protein in 5% SDS. Will these samples...

28 February 2021 7,370 3 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How to replicate the well conditions of a crystal hit with 20%(w/v) isopropanol?

I had a crystal hit in a well with 0.1M HEPEs pH 7.5, 10%(w/v) PEG 4000, and 20%(w/v) isopropanol. Why is isopropanol in w/v here if it's just solvent? How do I recreate this well condition?

25 February 2021 9,761 1 View

Adam B Shapiro

I would consider refolding and reconcentration to be a single step, so I would omit the 4th line and rename the last line.

Sadia Kanwal

yes Adam i thought the same....many thanks for your suggestion...

Dominique Liger

Hi there,

Just consider the final product to calculate the overall yield.