Hello

Here is the general step wise procedure for nanoprecipitation.

1. Dissolve your drug and polymer in a suitable organic solvent (Eg., Acetone, THF, ACN)

2. Prepare an aqueous surfactant solution of required percentage (Eg. 0.1-2% Tween 80, 0.1-3% PVA, Poloxamer 188 etc.)

3. To approx 10-20 ml of surfactant solution in a beaker, add organic phase (approx 1-2 ml) drop wise under stirring on a magnetic stirrer.

4. Continue stirring (overnight) till the organic solvent evaporates

5. Collect the nanoparticles by centrifugation

6. Characterize for size, PDI, and entrapment

7. Optimize the variables (drug to polymer ratio, choice of solvents and surfactants, percentage of surfactant, stirring speed etc.) to achieve desired results

PS: The procedure given is very basic and can help for initial trials. Each step might need further optimization.

All the best!

Hello

Here is the general step wise procedure for nanoprecipitation.

1. Dissolve your drug and polymer in a suitable organic solvent (Eg., Acetone, THF, ACN)

2. Prepare an aqueous surfactant solution of required percentage (Eg. 0.1-2% Tween 80, 0.1-3% PVA, Poloxamer 188 etc.)

3. To approx 10-20 ml of surfactant solution in a beaker, add organic phase (approx 1-2 ml) drop wise under stirring on a magnetic stirrer.

4. Continue stirring (overnight) till the organic solvent evaporates

5. Collect the nanoparticles by centrifugation

6. Characterize for size, PDI, and entrapment

7. Optimize the variables (drug to polymer ratio, choice of solvents and surfactants, percentage of surfactant, stirring speed etc.) to achieve desired results

PS: The procedure given is very basic and can help for initial trials. Each step might need further optimization.

All the best!

Dear

The PLGA nanoparticles can be able to prepared by a precipitation–solvent evaporation method. For instance, briefly, 75 mg of PLGA and 2.5 mg of a drug were dissolved or suspended in 5 ml of acetone. This organic phase was poured into 15 ml of water containing 75 mg of Pluronic F-68 with moderate stirring at room temperature. Nanoparticles were immediately formed, and acetone was then removed from the colloidal suspension by rotoevaporation under reduced pressure. The resulting particle suspension was filtered through a 1.0-micrometer cellulose nitrate membrane filter and concentrated to a final volume of 10 ml by removal of water under the same conditions. Before they were added to the organic phase, drug were dissolved in 150 microlitre of 0.1 N HCl adjusted with 0.1 N sodium hydroxide solution and water, respectively, to pH 4.0.

Dear Gill,

I prefer you to study https://www.springer.com/cda/content/document/cda.../9783319414195-c2.pdf?.. Link.

Best Regards