Hi Everyone.
I’m master student. I’m studying about stem cell differentiation, mostly in chondrogenic, osteogenic and cardiogenic differentiation. could Anyone help me with a protocol? I’m trying with one that I found but I don’t sure about on cortisol concentration.
I hope that someone can help me. Thanks.
#stemcell
#mesenchymalstemcell
#MSC
I would suggest that you ask your supervisor, who has assigned this project to you, to provide you with appropriate publications.
I used a kit to differentiate MSCs to osteogenesis and adipogenesis described in the enclosed reference. But chondrogenesis, I used method described in this paper. Cardiogenesis, I did not study. But you should find some publications on this too
A Simplified Method for the Aspiration of Bone Marrow from Patients Undergoing Hip and Knee Joint Replacement for Isolating Mesenchymal Stem Cells and In Vitro Chondrogenesis.
Juneja SC, Viswanathan S, Ganguly M, Veillette C.Bone Marrow Res. 2016;2016:3152065. doi: 10.1155/2016/3152065. Epub 2016 Feb 11.PMID: 27057356 Free PMC article.
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