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Questions related from Cristina Crespo
I am isolating PBMCs from whole blood by Density Gradient Centrifugation. have used Lymphoprep and a Leucosep tube, and after centrifuging the sample, the layers in the tube were more red than...
30 October 2024 7,431 2 View
I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...
15 July 2024 4,017 5 View
I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...
08 March 2024 10,024 2 View
Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!
08 January 2024 2,944 5 View
I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...
05 January 2024 7,879 0 View
I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...
03 January 2024 5,425 1 View
I am going to phenotype PBMCs with flow cytometry and I will fix the samples before running them. One of my fluorochromes is PE-Cy7 from BD Biosciences, and the vendor says not to use formaldehyde...
30 October 2023 7,226 1 View
