Cristina Crespo

6 Questions 6 Answers 0 Followers

Questions related from Cristina Crespo

Can ROBINS-I be used to measure risk of bias in single-arm studies?

I am doing a systematic review, and I am measuring risk of bias with RoB2 for RCT, and ROBINS-I for non RCT. My questions is, for single arm studies, can I use ROBINS-I? I am not sure how to...

15 July 2024 3,421 5 View

Should I block compensation beads with mouse serum in flow cytometry?

I am using mouse serum to block Fc receptors before staining, for flow cytometry. My question is if I should also add the serum to compensation beads before staining them, so that the cells and...

08 March 2024 9,919 2 View

Can staining for flow cytometry be done in flat bottom 96-wells plates?

Most staining protocols for flow cytometry in 96-well plates use V-shape or U-shape plates. I would like to ask if staining could also be done in flat-bottom plates. Thank you!

08 January 2024 2,855 5 View

Do FMO controls for flow cytometry need to be run in each experiment?

I plan to run my experimental samples in the flow cytometer in batches. My question is if FMO controls have to be prepared and run every time I run experimental samples, or if I could only do them...

05 January 2024 7,809 0 View

Statistical analysis for PBMCs immunophenotyping by flow cytometry?

I am immunophenotyping PBMCs by flow cytometry using markers for T cells to analyse the different subsets (Naive, CM, EM and EMRA), and to measure senescent and exhausted t cells. I want to...

03 January 2024 5,351 1 View

Should I use a stabilizing fixative for flow cytometry or any formaldehyde would do?

I am going to phenotype PBMCs with flow cytometry and I will fix the samples before running them. One of my fluorochromes is PE-Cy7 from BD Biosciences, and the vendor says not to use formaldehyde...

30 October 2023 7,152 1 View