How to pack empty column with zinc powder?

How do you pack empty column with zinc powder? This is for the Vitamin K1 analysis using HPLC.

26 October 2020 3,904 3 View

What is the suitable temperature to store Vit A, E, D sample extract extracted from milk powder?

I run the sample preparation using AOAC Vit A,E,D method, that require saponification, concentration , drying using nitrogen gas, reconstitute with methanol. Since the HPLC method development is...

23 October 2020 6,430 4 View

How to trim and manipulate a database of homologous functional gene's amplicons, cloned in a plasmid and Sanger's sequenced (F/R) ?

Hello, I'm looking for the diversity of homologous functional genes among 4 different environmental samples. To this end, I amplified them by PCR using degenerated primers (CODEHOP), cloned 96...

22 July 2019 7,975 3 View

Reason for vortexes on bottom of BN PAGE gel?

Hi all, I have run BN PAGE twice so far and am seeing distortion of the bands at the bottom of the gel. I use a 4-15% precast gradient gel. I load 15-20 ul of sample in every other well and...

04 November 2018 7,131 2 View

Immunisation that induces immune response but does not gives protection?

19 December 2016 8,221 4 View

How to extract RNA from caecum?

I am trying to find out the protocol to extract RNA from the caecum of the chicken. Thinking logically, the condition of the caecum (or even any parts of the intestine) provides a great source of...

02 October 2016 5,754 1 View

Which is better to detect protein-RNA binding, RNA-IP or RNA-CLIP?

19 April 2016 6,578 3 View

How can digital ways of learning and teaching be embedded in curriculums to support the development of digital graduate attributes?

I'm looking for ways to bridge digital divides on campus between those with digital confidence and those who are more digitally reluctant or resistant - who I call the digitally shy. Digital...

28 March 2016 9,966 3 View

How might we justify high backgrounds in indirect ELISA?

Hi everybody,We are doing some indirect ELISAs as part of our project; however we are having some problems with what it is supposed to be a negative control.Here are some details about our ELISAs,...

08 September 2015 1,627 22 View

How do you gate naive T cell population?

Hi I was wondering if anyone can shed some light on this problem I'm stuck with. (Please check the attached PDF file) [Background] I isolated naive T cell (or what I thought to be) using a...

26 July 2015 5,021 4 View

Which and How to use teaching strategies and procedures to effectively integrate the four skills while teaching English Language Online?

As an inexperienced teacher in the field of online teaching, I have the fear of unconsciously ignoring one of the skills while focusing on not ignoring the other! Therefore, I wish to make use of...

09 February 2021 5,101 4 View

Does good inferential comprehension first require solid literal comprehension?

Hello, This can be a silly question to ask, but my main field is not in reading comprehension. While I try my best to phrase my question, it can sound a bit awkward. Thank you for your...

11 November 2020 811 3 View

How are the 3 levels of reading comprehension reflected in grade-level English Language Arts curriculum?

Hello, How are the 3 levels of reading comprehension (literal, inferential, and critical) reflected in grade-level English Language Arts (ELA) curriculum? In particular, normally, is literal...

01 November 2020 1,240 4 View

Is the level of cognitive flexibility positively correlated to the level of reading comprehension?

Hello, Is the level of cognitive flexibility positively correlated to the level of reading comprehension (greater cognitive flexibility brings higher level of reading comprehension)? If there is a...

01 November 2020 8,254 5 View

Is there a reading comprehension theory that suggests simple reading questions are best solved via simple text analysis, and vice versa?

My main field is not Linguistics/reading comprehension so what I am asking might sound a bit silly or not make a lot of sense. My question could certainly be better-phrased. Is there a theory in...

23 October 2020 8,310 2 View

First time project advice: How to close transcription factors, SOX9 and NFIB, into baculoviral vector, pEZT-BM?

Hi all, I am new here and I am about six weeks in to my first ever research project! (I just came here to kick-start my career as a researcher.) :) The aim of my research project: "The aim...

15 September 2020 9,890 3 View

Could you collaborate, please?

Hello everyone! I've developed a 35-item questionnaire to measure ESP students' critical reading strategies awareness while reading materials in English (as part of my Ph.D. research). Now, I need...

27 June 2020 2,484 3 View

How do you read the papers without falling into a rabbit hole?

Often while reading a paper, I will see a citation that that seems more relevant than my current one. I'll switch to the new paper, only to experience a similar feeling. Or maybe I need to look...

11 May 2020 3,883 23 View

How to compute PISA 2018 tests scales?

Good Evening, The results of the PISA 2018 test were published at the end of 2019 on the OECD website (https://www.oecd.org/pisa/data/2018database/). Among the available data are the Code book...

26 February 2020 7,507 3 View

Is it worth to study the effect of item format on psychometric characteristics of the items?

In my work I would like to study the effect of item format on psychometric characteristics of the items in reading comprehension assesment. At the moment, feedback concerning the theme of my...

15 December 2019 9,249 3 View