I would like to extract a plasmid from Desulfovibrio fructosovorans?

15 April 2021 0 8K Report

Hello,

I would like to extract a plasmid from Desulfovibrio fructosovorans. I have tried many protocoles (boiling, lysing,...) but in some cases I extract all genomic DNA (I have a smear on my gel) and in other case I extracted nothing.

Is anyone have a good protocol to purify plasmid from this kind of bacteria (Gram - and/or SBR) or have any advice to help me?

Thanks

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