Spontaneous/chemical induced mammary tumor model through alteration of metabolism?

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How to carry out survival analysis of a one time use machine which is stored and tested at regular intervals for functionality?

I want to carry out reliability study of a machine with various sub components both electrical and mechanical. The machine is a one time use (similar to a decoy or rocket) and is stored at...

30 June 2024 2,435 0 View

Is there any home portable biowaste manager ?

home based bio waste management

02 June 2024 8,590 4 View

How to develop a flat panel display glasses ?

Dear friends, I am looking to develop a new project for the applications of displays using float glass. How and what process is needed to develop such flat panel displays what are the ideas and...

09 May 2024 3,233 0 View

How do we control pin holes in the coated thinfilms by using large area magnetron sputtering techniques?

Dear ResearchGate colleagues, professors, and scientists, I'm encountering challenges with coated thin films produced using a sputtering system. Following treatment through tempering at 650...

11 February 2024 3,585 4 View

PLGA Microspheres. Alcohol or ester terminated polymers?

I have read a research paper "Development of a novel morphological paclitaxel-loaded PLGA microspheres for effective cancer therapy: in vitro and in vivo evaluations". I want to synthesize PLGA...

13 December 2023 9,857 1 View

Hello everyone, I am working on medicinal plant. How can i perform Principal components analysis?

I have done metabolite fingerprinting of plant extract using LC-MS and GC-MS.

29 November 2023 4,511 3 View

Are Geochemical Weathering and Pedochemical Weathering distinct processes?

They seems synonymous terms describing the same phenomenon.

24 October 2023 4,332 0 View

How to Deposit titanium nitride layer with x varies from 0.8 to 1.2 using sputtering coating ?

I am looking to deposit a single-layer titanium nitride layer with a sheet resistance of approximately 60 ohms and a layer of TiNx (x value should be in the range of 0.8 to 1.2). The developed...

22 October 2023 7,594 4 View

What is the name open source software for LC-MS data analysis?

Please mention name of open source software

02 October 2023 2,301 3 View

Why is the acceleration due to gravity (g) not taken into consideration when calculating the weight of soil on a one-hectare area?

Weight of soil = Area of soil * Bulk density of soil * Depth of soil. where mass=density*Volume; volume=Area* Depth. However the formula for weight in physics is mass*accelation due to gravity.

22 August 2023 9,430 5 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Weak DAPI staining after immunohistochemistry - how to improve?

After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...

05 August 2024 9,637 2 View

The Curse of Evolution and Complexity?

Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...

05 August 2024 6,247 3 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View