Can anyone suggest me what is the concentration of Acridine orange to be used for spheroid labelling and for how much time?
Article Acridine Orange/exosomes increase the delivery and the effec...
Article A simple, low-cost staining method for rapid-throughput anal...
There are many different models that can be used to model beach profile evolution, one of the critical factors in this fixing the direction for the sediment movement. This will determine if a...
02 March 2023 6,413 0 View
The fortran code given in the book by griffiths and Smith "Programming the finite element method " 5th edition. As after defining the free surface and calculating pore pressure as product of...
28 May 2022 7,912 0 View
Hi everyone I am working on Stevia rebaudiana. I have gone through few research articles where water, methanol and ethanol have been used for the extraction procedure. Can anyone suggest that...
15 February 2022 6,562 5 View
I am a postdoc student. I have evaluated miR expression in healthy controls and cases. I want to perform ROC curve analysis which I did using GraphPad prism which gave me the following: AUC, SE,...
26 September 2021 1,174 3 View
When I was doing a SDS-PAGE upto 25kD the bands of the protein ladder got resolved properly , below the 25kD the 20, 15 and 10kD bands did not resolved and the tracking dye of the samples was...
12 April 2021 8,845 6 View
Need to predict the crack propagation phenomenon in a CI component. Suggestions are needed regarding the the methods ( XFEM, J integral, etc.,) which will predict the crack propagation behavior...
06 February 2021 2,150 4 View
I have the yield stress & ultimate stress values for Al alloy. But I don't have the percentage of elongation. Using Yield & ultimate , is it possible to calculate the the % of...
22 December 2020 1,916 1 View
Can a change in relative humidity cause unfolding of proteins inside sealed containers when stored for a long time (say 8 to 12 weeks) at room temperature? Any insight and/or literature...
16 December 2020 5,813 1 View
Hello, I have been trying to simulate the active groundhook system in Matlab/Simulink. The force equation for the groudhook actuator force is given by F_gnd = -c_gnd*vel_wheel, where c_gnd is the...
25 September 2020 4,334 3 View
I want to convert the Young's modulus and Poisson ratio of an orthotropic material given in Cartesian coordinates into cylindrical coordinates. Please suggest me the way or provide me the link or...
15 July 2020 3,915 5 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
I have a Polyjet that has passed its labeled expiration date for 1 year and I'm going to re-start transfection experiments. It is really needed to change it? Transfection efficiency may be lower?
23 July 2024 8,059 1 View
The above are manually labeled extrinsic matrices based on the first image It can be seen that the projection error at the edge is large, while the error at the center is small. What could be the...
23 July 2024 7,479 3 View
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hello everyone. During my analyses on LC MSMS, the area ratio (Standard Area/Isotopically labelled Standard Area) increases with time for the quality control point. I'm having trouble explaining...
08 July 2024 3,150 2 View
The image shows 2 western blots. The first one is of protein A, and the second is for the flag tag that the protein is bound to. The lanes are as follows Ladder, Beads, SAB(unbound), and elution....
01 July 2024 3,183 0 View
Hi, I´d like to trace the C and N flow in a recirculating aquaculture system by labelling the fish feed with concentrated stable isotopes. From a supplier, I found a product ouf of algae lysate...
19 June 2024 746 0 View
My goal is to see two kinds of particles mixed well in an aggregated form. My experiment was as follows: 1. Prepared Microparticles 1 + EDC/NHS + RhoB, and Microparticles 2 + EDC/NHS + FITC. 2....
19 June 2024 2,680 1 View
During electrocatalytic nitrogen reduction to ammonia, we perform isotopic labelling test using HNMR to confirm source of ammonia.
29 May 2024 5,165 0 View
I have FITC labeled proteins, and I would like to detect its fluorescence after running proteins western blot. Does fluorescence quench in WB condition? so I can detect FITC labeling (without...
27 May 2024 4,087 3 View