What is the size of human IFNAR2 (interferon alpha receptor 2) protein?

While I found a prominent 58kDa (others 37.4, and 27.3 kDa forms) from data banks, Domanski et al 1995 JBC presented a 110 kDa isoform. I am not sure about this really. Please share your expertise...

01 February 2018 6,511 2 View

Detecting specific antigen to an unknown Antibody?

If I have an antibody enriched in a sample and I would like to know which antigen to employ, what techniques can I use? Do you think methods such as co-IP coupled with mass spec analysis is the...

31 December 2012 820 18 View

Optimal condition for Thrombin cleavage reaction

I have a fusion protein separated by thrombin cleavage site with GST. I eluted my fusion protein with 20mM Glutathione(reduced),50mM Tris(pH8),500mM NaCl,10mM CaCl2,10% glycerol and 5mM 2-ME. This...

11 December 2012 7,456 16 View

Immunoblot: House keeping genes for PBMC and Macrophage cell types

I would like to check the expression profile of some proteins of PBMC and PBMC derived macrophages(with treatments). For the same purpose, what would be a good HKG for western blot? Any of your...

11 December 2012 2,565 3 View

DNAse I digestion to kill residual vectors

To avoid the carry over of plasmids with virus genome used in transfection, we treat the virus supernatant with DNAse I. In what way I can increase the efficiency of the reaction? Is the DNAse I...

10 November 2012 1,438 21 View

"Need of CaCl2 in protein purification buffer"

Why do some protocol uses CaCl2 in the lysis and elution buffers while purifying the soluble protein from E.coli?

10 November 2012 4,122 2 View

Real-time PCR primer designing software?

I would like to make primers for my qPCR. For that, I need a reliable software. I know the (ABI) Primer express software(paid) is preferred, but do you know any freeware that does the job well?

08 September 2012 7,803 12 View

Casein versus BSA

Why do we need BSA as a blocking buffer for certain antibodies? What is different from casein? Thanks, Anand

05 June 2012 455 4 View

"Protein aggregation after IMAC elution" :(

I have some problem with my protein purified via IMAC(native condition), after my IMAC purification, when I try to concentrate eluates, considerable amount of protein start aggregates! I tried...

11 December 2011 3,164 7 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Theoretical computer virology ? is still alive ?

HI Everyone! I am asking to know by you, expert in cybersecurity and mathematics, if Computer virology (the one of Cohen and Andler) is still an active field of research. That research made in...

08 February 2021 7,613 6 View

Serum causes Hemagglutination in HI test. Virology Expert Please help how to resolve this issue while doing HI test for NDV and Avian Influenza?

I am doing HI Test for NDV and Avian Influenza Viruses but before going to start I first tested the serum whether any reactivity exists or not with 1% washed Chicken RBC. but I found that some...

11 January 2021 1,043 7 View

Can you trust vaccines that were made in a year, will help fight Sars-Cov2?

That vaccines have been produced in such a short time, makes me doubt if they will help fight this pandemic and if they will not do us more harm.

06 January 2021 3,469 19 View

Any papers discussing the effect of Zika virus on neuron development and its effects on neuronal functions?

I am looking into this research, any interesting papers relating to this would be amazing. Papers on pregnant women and infants would be great. Thank you :)

30 December 2020 1,962 3 View

Can only the spike protein of the virus provide immunity?

Virology, microbiology, immunology, vaccine

21 December 2020 424 16 View

How can chemistry help fighting COVID-19?

Chemistry is frequently termed the "central science". As such, chemistry is expected to play a major role in fighting the coronavirus. For example, hand sanitizers and drugs are all based on...

30 November 2020 4,983 17 View

What is the scope of doing PhD in Virology in the UK?

With the great interest in Virology in the world due to the COVID-19 pandemic and its related vaccine development is doing a Phd in Virology from a good institute in UK the right thing to do?

26 November 2020 3,681 3 View

Who can collaborate with us?

Salam (Hello)! We have prepared some new compounds with important potential to inhibit viruses. We are interested in inhibition of Hepatetis-B/C, Nipah, Nil and coronavirus. Who can collaborate...

22 November 2020 3,850 14 View

Biotech Startup Research?

26 October 2020 9,698 5 View