What is the size of human IFNAR2 (interferon alpha receptor 2) protein?

More Ananda Ayyappan Jaguva Vasudevan's questions See All

Detecting specific antigen to an unknown Antibody?

If I have an antibody enriched in a sample and I would like to know which antigen to employ, what techniques can I use? Do you think methods such as co-IP coupled with mass spec analysis is the...

31 December 2012 820 18 View

Optimal condition for Thrombin cleavage reaction

I have a fusion protein separated by thrombin cleavage site with GST. I eluted my fusion protein with 20mM Glutathione(reduced),50mM Tris(pH8),500mM NaCl,10mM CaCl2,10% glycerol and 5mM 2-ME. This...

11 December 2012 7,456 16 View

Immunoblot: House keeping genes for PBMC and Macrophage cell types

I would like to check the expression profile of some proteins of PBMC and PBMC derived macrophages(with treatments). For the same purpose, what would be a good HKG for western blot? Any of your...

11 December 2012 2,565 3 View

DNAse I digestion to kill residual vectors

To avoid the carry over of plasmids with virus genome used in transfection, we treat the virus supernatant with DNAse I. In what way I can increase the efficiency of the reaction? Is the DNAse I...

10 November 2012 1,438 21 View

"Need of CaCl2 in protein purification buffer"

Why do some protocol uses CaCl2 in the lysis and elution buffers while purifying the soluble protein from E.coli?

10 November 2012 4,122 2 View

Real-time PCR primer designing software?

I would like to make primers for my qPCR. For that, I need a reliable software. I know the (ABI) Primer express software(paid) is preferred, but do you know any freeware that does the job well?

08 September 2012 7,803 12 View

Casein versus BSA

Why do we need BSA as a blocking buffer for certain antibodies? What is different from casein? Thanks, Anand

05 June 2012 455 4 View

Specificity and the action of Subtilisin A

Hi, does any one has experience with Subtilisin A for the digestion of Viral core/membrane protein or any other? please share your knowledge.

31 December 2011 3,611 11 View

"Protein aggregation after IMAC elution" :(

I have some problem with my protein purified via IMAC(native condition), after my IMAC purification, when I try to concentrate eluates, considerable amount of protein start aggregates! I tried...

11 December 2011 3,164 7 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

Have someone successfully used the ER alpha (Santa Cruz sc-8002) antibody in immunostaining ?

Santa Cruz now recommends this new antibody (F-10) but I can't find a publication using this reference. I'm working on human et non human primate hypothalamus and so far, it doesn't work. Is...

02 March 2021 10,003 2 View

Energy minimization in LAMMPS using NVT ?

How do I do energy minimization in lammps with NVT ensemble ? I am using the following command and it does not seem to work. It stops at extremely high energy and gives me the error zero search...

01 March 2021 2,294 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How does an antibody have full intact antibody + half antibody?

Hello, I was just wondering how does a antibody have full intact antibody + half antibody? Is it because of the drug conjugation?

28 February 2021 1,577 1 View

Does anyone use antinuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples?

I am looking for anti-nuclear antibody (ANA) and anti-Phospholipid antibody ELISA kit for Non Human Primate serum samples. Any suggestions highly appreciated. Thank you.

28 February 2021 6,512 3 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

Daniel Patiño-García

Dear Ananda,

According to UniProt, the human IFNAR2 length is 515 and it has a mass of 58 kDa (http://www.uniprot.org/uniprot/P48551).

Best regards.

Ananda Ayyappan Jaguva Vasudevan

Daniel: (As given in my question) I have checked the data banks including NCBI, ensembl, gene, uniprot, and other you can imagine .

My question is specific and targeting the researchers who have worked before with the same!