Specific marker for exosomes isolated from adipocytes?

More Nanthini Jayabalan's questions See All

Is it possible to administer drug dissolved in ethanol orally ?

I wanted to give Cholecaliciferol (vitamin D) dissolved in ethanol orally to Wistar rats aged 5 to 6 months. Majority of the research articles suggests Subcutaneous route however I would like to...

27 September 2021 9,689 3 View

Proteomics to track/ trace origin of exosomes?

Has anyone performed proteomics to determine the origin of exosomes in plasma? Can you please explain to me how did you performed it? Thanks :)

30 October 2017 4,433 2 View

CD31 band at 55 kDa?

I performed Western blot for CD31 marker with expectation to observe band at ~130 kDa. However, i observed bands at 55 kDa for all my samples. According to supplier, additional bands are usually...

14 November 2016 4,235 2 View

Iodixanol gradient vs sucrose gradient

Can someone please enlighten me on why some prefer to use iodixanol gradient compared to sucrose gradient? Is this dependent on type of cell that is being used? Are there any major differences...

21 August 2016 9,557 3 View

Why the probability of electron density of 's' and 'd' orbitals is differ?

why the probability of electron density for 's' orbital is in spherical shape and for 'd' orbital not in spherical spherical shape?

16 August 2016 5,034 2 View

CCI or FPI to study the basal forebrain (BF)?

Hello everyone. We are intending to study the effect of TBI on BF neurons. I did some literature search and found both FPI and CCI model have been used for this purpose. Between these two models,...

07 June 2016 7,140 2 View

How to choose a solvent to dissolve a crystal for liquid crystal display?

we are trying to dissolve a crystal for liquid crystal display and would like to know which solvent to choose.

25 February 2016 275 2 View

Can any one provide thin section photographs of melilite bearing lamprophyre or alnoite?

We had encountered a small dike with evident porphyritic texture in both hand specimen and thin section scales. The euhedral minerals are colorless, tabular or elongated tabular some times wedge...

11 March 2015 1,188 32 View

Are there any (online or offline) tutorials for GCDkit scripting in 'r' language?

GCDkit is one of the most versatile packages for igneous petrology. Writing scripts in 'r' for GCDkit has a steep learning curve. Tutorials may help to facilitate learning with feedback.

10 July 2014 144 12 View

What is crustal delamination and how does it influence the petrogenesis of alkaline rocks, e.g. lamprophyres?

Analytical data of lamprophyres (minette and spessartite) display a primitive character and are enriched in LILE with depleted HFSE. The geotectonic environment as suggested by trace element data...

04 July 2014 2,508 8 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

Does anyone have issues using Prepman Ultra reagent for MicroSeq ID bacterial, fungal and yeast sample preparation?

I have been attempting to extract DNA from Bacterial, Fungal and Yeast banked samples (>1e7 cells) using Prepman Ultra reagent and I seem to be struggling to obtain a sequence. Although the...

01 August 2024 2,079 0 View

Is it common for heterotrophic bacteria to grow on Pro99 and SNAX media?

I am attempting to isolate picocyanobacteria from seawater (pre-filtered with a GF/D membrane) using the pour plate technique and filter plating method (Kearney et al., 2022) on Pro99 and SNAX...

30 July 2024 1,040 1 View

Question about MHB (Mycorrhiza Helper Bacteria) isolation technique??

What are the most effective isolation techniques for obtaining MHB?? Is there are best approach techniques? I keep struggling in obtaining it.

26 July 2024 7,387 0 View

Question about Single Strain Arbuscular Mycorrhiza Fungi??

Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...

26 July 2024 6,520 1 View

I found the zeta potential of exosomes in DLS and it was -8mv. So is it correct or not ?

What should be the zeta potential of bovine milk exosomes

25 July 2024 7,376 5 View

Isolation of PLV6-Bmal-Luc plasmid?

I am currently at a stage in my research where I will be using the pLV6-Bmal-luc plasmid (Addgene #: 68833). However, I am encountering some issues with the isolation of this plasmid and am unable...

23 July 2024 5,296 0 View

Can I use sonicator only to lyse the exosomes and measure their protein contents using BCA assay kit?

Hello, I want to ask regarding exosome protein quantification method. Is it necessary to lyse exosomes before measuring their protein contents using BCA assay kit? Or can I just straight away...

23 July 2024 4,603 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View