Can someone please enlighten me on why some prefer to use iodixanol gradient compared to sucrose gradient? Is this dependent on type of cell that is being used? Are there any major differences between these two, in terms of end results?
Hi, please go through this: https://www.biolynx.ca/tech/articles/axs_tumorcells.pdf
Sucrose gradient: you have to make it manually by preparing various density sucrose solutions, whereas iodixanol gradients form by itself if given some time(http://www.ncbi.nlm.nih.gov/pubmed/12805920). That makes the gradient making process simple. As long as you keep the gradient to sample ratio constant (also protein concentration of sample), you can get reproducible results in iodixanol gradients. It is also iso-osmotic to some organelles such as peroxisomes. Also the range of iodixanol gradient is wide so it can also be used for virus concentration.
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