Dear all,

I am trying to find an alternative to southern hybridization for copy number detection in transgenic Arabidopsis. I have attempted the QD PCR protocol (T.Kihara ) for the protocol but I am unable to really understand its rationale.

If anyone can provide me an alternative for copy number detection or provide me a simplistic reasoning behind the gel images from QD PCR, it would be of great help.

Thank you!

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