Giorgia, Can you specify the target fungi, please?

It is global forAscomycetes and Basiodiomycetes.

Hi

You can use some of these primers: chit31, chit33 or chit42.

34 cycles

94*C 90 seconds

56*C 30 seconds

72*C 45 seconds

at last 72*C 4-7 minutes.

You can use attached file for primers.

good luck

Thanks so much.

@Aydin Hassanzadeh the list of the primer you added, it is for fungal chitinase?

Yes, it is. It is for fungal chitinase.

@Aydin Hassanzadeh Thanks so much for this information.

You're welcome.

@Aydin Hassanzadeh is possible for you write me the reference for these three primer for fungal chitinase?

Hi

Sorry for delay

You can find some paper on google scholar.

One of the attached files is in Persian (in this paper had been used all 3 primers)!

Good luck

Don't worry. Thanks so much your answer.