Why does it use TE as negative control in qPCR multiplex?

Dear all, I have a question. I do not understand why does it use TE buffer as negative control in qPCR multiplex? Is it the same if I use the mix as negative control? Thanks so much Giorgia

06 July 2018 6,969 2 View

Someone use this primers GH20_340F/GH20_440R?

Hi, I try a PCR with these two primer to amplify the chitinase gene for the fungi. I try more different Tm, concentration of primer, concentration of DNA, but I did not obtain bands. Someone have...

03 April 2018 2,888 11 View

Protocol PCR primers narG1950m & narG2050m?

Did someone used these primers in PCR or qPCR? Because I have a problem to perfom the PCR and I obtained the no appropriate band in the samples and NTC. Thanks Giorgia

31 December 2017 8,664 3 View

Did not see amplification in ITS qPCR?

02 March 2017 6,751 4 View

RT-qPCR protocol one or two steps?

Is best to prepare the RT-qPCR in one-step or two-step?

10 November 2016 5,669 9 View

Is BEST analysis (PRIMER 7 software) a multivariate analysis?

I try to analysis the best correlation between the biotic and environmental dataset. My question is: if I have one variable for the biota dataset and more variable for the environmental dataset,...

01 February 2016 1,753 2 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Genome-wide gene identification protocol ?

Is there any book chapter/book, webpage or research article available to understand genome-wide gene identification?

28 February 2021 8,095 1 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

How to add missing citation?

Dear colleagues, I have found that many authors have a concern regarding their missing citation in RG because often citation in RG is lower than google scholars. I also missed some citation...

26 February 2021 928 6 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View

What is the most suitable medium of ascomycetes fungi ??

What is the most suitable medium of ascomycetes fungi ??

26 February 2021 4,413 1 View

Centrality measures computation in Biblioshiny/ Vosviewer?

26 February 2021 7,279 2 View

RNA-seq DEGs downstream analysis and reporting in literature?

I used EdgeR to get the list of DEGs (differentially expressed genes) from my liver RNA-seq experiment. I have done the gene overrepresentation and GSEA based pathway analysis. I am currently...

23 February 2021 8,969 1 View

Could you please suggests a tutorial or paper or any other materials to learn multiple gene expression in TCGA and GTEx databases?

I would like to analyze multiple gene expressions in normal and cancer tissue by using the data in the TCGA and GTEx databases. Please suggest a tutorial or paper showing step by step analysis of...

23 February 2021 9,007 3 View

Are there any databases for gene co-expression or expression pattern clustering?

I am currently working on gene clustering based on co-expression pattern in mouse brain. The problem is I do not have some solid way to test my result. Are there any suggestions for databases...

22 February 2021 4,374 3 View