Someone that had to use the 8-isoprostanes immune assay kit (CAYMAN), know how to prepare the phosphate buffer?

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My Xenopus oocytes (healthy frogs) explode before I can make any electrophysiological records, why?

Xenopus laevis oocytes are used in electrophysiology to make transient expression of injected mRNA fragments. Through the technique of 2-electrodes voltage-clamp we can record the physiological...

11 January 2021 6,005 4 View

What could be the reason of no transformation using a ligation mixture?

Recently, I have been working with some genes contained in pBlueScript II SK (+) and that contain XhoI and XbaI restriction sites. I would like to clone them in PET28a. For instance, the...

22 June 2020 3,474 5 View

How to install YAML.pm?

I'm trying to annotate a yeast's genome using FunGAP but it stopped in the run4 of Maker. The gmes log said that I may need to install YAML module, as follows: Can't locate YAML.pm in @INC (you...

26 November 2019 7,121 2 View

Do you want to collaborate in a review about MOFs on electroanalysis?

Hi. I'm writting a review about the application of MOFs to desing modified electrodes for electroanalytical application, specifically carbon based electrodes, so I'm looking for collaboration to...

02 May 2019 9,900 2 View

Someone know how to prepare cocoamidopropylbetaine with solid content over 40 %?

i have been trying to prepar cocoamidopropyl betaine with solid content above 40 % but i only have reached 37 % of solids. Every time i try to increse the solids content it forms a gel.

10 April 2018 2,318 1 View

How do I perform calculations on data under a detection limit?

I have values of ion concentrations from NH4Cl soil extracts. Many of the values I have are below the detection limit and I still need to perform calculations to get units of meq/100 g of soil....

06 February 2018 7,429 3 View

A good p-IR antibody for WB?

Hello! I work with mouse islets and I want to detect phospho-Insulin Receptor by western blot but all the antibodies I have been testing are horrible... even with amounts of protein around...

25 January 2018 9,101 2 View

Metagenomics databases. Which one choose?

In bioinformatics databases play an important role in the results, whether chimera detection or OTUs assignment (16S metagenomics). In your experience, which databases have a better relation...

11 January 2018 858 3 View

I have a type I collagen sample in acetic, obtained from pig skin. It is a gel. How can I make it soluble to run an SDS gel?

How do I separate the proteins that make up the triple helix? Nor can I measure proteins by Bradford for the same solubility problem. I appreciate suggestions

26 December 2017 1,373 6 View

Does anyone has a protocol for determination of minimum lethal dose (MLD) of toxins for mice?

I was looking for a protocol to calculate the MLD in mice but I didn't find it. Thanks for yout help. Robert

28 September 2017 2,954 1 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View