Someone that had to use the 8-isoprostanes immune assay kit (CAYMAN), know how to prepare the phosphate buffer?

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Personal recommendations for GAPDH antibody?

Hello! I am planning a western blot experiment, and I am looking for GAPDH antibodies to order. In the past, I have experienced a lot of hit-or-miss when ordering antibodies based on just the...

07 July 2024 1,685 0 View

PVDF or nitrocellulose to visualize phosphorylated proteins?

Which membrane material is the best for capturing and visualizing phosphorylated proteins in a western blot? Or is there no difference?

05 June 2024 9,502 2 View

Best primers for human genes COXI and COXIV?

Hello everyone! My PI would like me to measure the amount of mitochondrial DNA in multiple cell lines using qPCR targeting COXI and COXIV. However, I have never done a qPCR that measures...

03 June 2024 6,795 1 View

Best apps for RNAseq heatmap generation in Illumina BaseSpace?

Hello! I am trying to analyze some RNAseq data I recently received. I would like to craft a heatmap comparing the expression of specific genes across my cell samples, but I am not sure which of...

08 May 2024 5,665 0 View

What is the best way to normalize p-S6(Ser235/236) protein expression?

Hello everyone. I am in the middle of performing western blotting experiments measuring expression of p-S6(Ser235/236) protein in total cell lysate. Originally, I was quantifying my data using...

11 April 2024 4,457 1 View

15 mL conical tubes & Eppendorf tubes compatible with liquid nitrogen and dry ice?

Hello! I need to snap freeze cancer pellets to submit for multiomics. Does anyone know of 15 mL conical tubes or Eppendorf/microcentrifuge tubes that can withstand liquid nitrogen and dry ice?

15 February 2024 4,199 1 View

How do I know if my sample is reduced or non-reduced for western blot?

Hello! I am not sure whether or not my protein samples are reduced or not. Based on the information below, can someone please let me know if they are reduced? Sample setup: __ uL total cell...

12 February 2024 4,912 1 View

I can't make this solution. I need help with the concentrations?

Hello all! I need to make 100 mL of the following solution. I tried to use the C1V1 formula, but I have dyscalculia so my math skills are non-existent. Ponceau-stained membrane can be detained...

01 February 2024 8,962 1 View

Can convergence (SCF) be improved for transitional metals?

In Guassian, which convergence algorithm is best for working with transition metal interaction? It is taking a long time to even get the first block of coordinates, in the output file it said to...

28 January 2024 4,276 1 View

Recommendations for protein markers of cell stress/starvation?

Hello everyone! I'm currently running an in vitro experiment where I test the effects of treating human cancer cells with "starvation media" (aka DMEM media with no FBS) on mTORC1 signaling. I...

18 January 2024 4,365 1 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to improve plaques in Double Layer Agar Assay?

Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...

06 August 2024 5,801 4 View

Why I can't see any band in SDS-PAGE?

Currently, when I run SDS-PAGE, I don't see any bands at all, even though I used the same material just a day ago and it worked fine.... In our lab, we dilute the 10X running buffer to 1X and...

06 August 2024 5,373 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,783 2 View

What is the best blank for nanodrop if I want to read a recombinant protein concentration?

Is it the "elution buffer" or the "dialysis buffer"? Note: I'll be using NanoDrop OneC

01 August 2024 967 3 View

EDTA buffer solution?

Hello everyone! How can i prepare EDTA buffer solution (pH 8.2) to determine GSH levels? Thank you in advance.

29 July 2024 4,374 1 View

Whether i can preserve leaves samples in 2.5% glutaraldehyde in 0.05 M phosphate buffer, pH 7 for coming several weeks for further LM study ?

I want to preserve the sample leaves for the LM study of the stomata and conidia analysis. Whether 2.5% glutaraldehyde in 0.05 M phosphate buffer with pH 7 can be applied for preservation.

29 July 2024 8,560 0 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Can a shoot-through event of a tri-state digital buffer cause momentary Hi-Z state?

// interested in the difference between floating events and short circuits.

22 July 2024 6,565 0 View