Does anyone have experience on the PhiX concentration required on NovaSeq for ddRAD or RADseq data?

Dear all, we have been running multiple ddRAD libraries prepared by following the protocol of Petersen et al 2014 in our lab on the HiSeq2500 (SR 100) in the past couple of years. We pooled...

28 September 2020 2,297 1 View

Read counts from RNA-Seq paired end reads?

Hello, I have the following question on paired end reads from RNA-seq: To get the total number of obtained reads from paired end reads (2 fastq files are generated (R1, R2), is it correct to add...

01 September 2020 7,715 5 View

Does anybody have experience with the detection of a FLAG epitope on a Western blot with the Sigma Anti-FLAG antibody M2 after stripping the blot?

13 August 2020 8,401 3 View

How can I test if my TaqMan Probe is annealing?

Hello! I'm trying to perform a TaqMan qPCR to check for certain fish species in environmental DNA samples. I am using a published primer + probe set. The probe is carrying FAM at it's 5' end and...

14 April 2019 4,907 15 View

What is the best method to extract RNA (including miRNA) from plasma if it is needed for NGS RNA sequencing?

Dear all, Few years ago, I used miRCURY Biofluids from Exiqon for extracting RNA from plasma for detecting miRNA by qPCR. However, this product is currently discontinued since Exiqon is now...

23 May 2018 6,880 4 View

Stats for dissertation?

I need some guidance on selecting the appropriate statistical methods for my research. I am investigating lone violent extremists by subtype (far right, far left, Islamic and single-issue) on 4...

11 May 2018 1,809 3 View

If I have cultured keratinocytes expressing only K14 gene, is it possible that under certain conditions, they become activated and express K16?

18 December 2014 1,476 6 View

How I can obtained well formed Embryoid Bodies from Embryonic Stem Cells in Serum free medium?

Hello Everyone I tried to differentiate Embryoid bodies (EBs) from mouse Embryonic Stem cells (E14tg2a) following the protocol I attached. However i failed during the process because despite cells...

01 January 1970 155 6 View

Looking for a psychometric assessment tools to measure maturity in adolescents?

Working on a behavioral therapy protocol and looking for a psychometric assessment tool to measure maturity in adolescents and young adults up to 24 years in age.

01 January 1970 3,684 7 View

How to perform Microsatellite data analysis?

Hello, I have an excel sheet with microsatellite data, I would like to make a NJ tree out of it. I have a basic level in bioinfo. Can someone please guide me through the steps to get the...

01 January 1970 6,828 2 View

How to analyze a particular region of my simulation box in GROMACS?

Hi, I am using GROMACS 5.1.4 to simulate water in contact with a surface. I am only interested to calculate some properties of water near the surface. Let's say at a distance of x nm from the...

03 March 2021 9,989 2 View

How to get onecolumn document from standard IEEE ACCESS LateX template?

I have prepared a manuscript in IEEE Latex template, but for some purpose, I need to prepare a word file as well (detailed format is not required). Compared to two column .tex file, one column...

02 March 2021 1,830 3 View

How can the modification of garden soil cause global warming?

Hello, I am working on a soil project and I found a primary research article that tests how the modification of agricultural soil can cause global warming, using a thermal collector, however I...

02 March 2021 7,161 3 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know to clarify these changes?

01 March 2021 1,290 2 View

How can I convert .vesta file to a .cif file?

I've used the "export data" option in Vesta software to convert my unit cell into a cif file however when I use that cif in atomsk it shows error such as "can't read the position of atom 0". If I...

28 February 2021 4,966 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

No title

25 February 2021 5,713 1 View

Anyone that can help with Maxquant TMT16plex which is missing?

Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...

25 February 2021 1,675 3 View

How to search for crystal structure for material with atomic position labelled?

I am using the crystallographic open database and materials project to search for cif file but the exact structure is not mentioned or how can I deduce that the given structure and atomic position...

23 February 2021 6,723 2 View