Software flowjo and flow cytometry. How to use?

More Fernanda Thomazini's questions See All

Does someone have tips on how to reduce the amount of entrapped oil in the membrane of GUVs with the Phase Transfer Method?

I'm trying to prepare GUVs using the phase-transfer method but apparently I'm getting residual oil entrapped in the bilayer. Did anyone manage to overcome this? Basicaly, I'm following the...

24 November 2020 2,962 1 View

How do you delete a Google Scholar profile?

For some reason, I have two slightly different names associated with my Google Scholar profile. Apparently, both names are associated with the same email. I tried to eliminate one of the names,...

21 October 2020 9,396 3 View

Using facebook for surveys?

Hi, Has anyone had any good (or perhaps bad experience) using a "boosted" survey on Facebook? Has anyone been able to publish using this channel for data collection? Or has anyone been...

12 October 2020 6,697 4 View

Someone has a suggestion to improve the purification of MMLV-RT protein?

I am working with a MMLV-RT (Moloney murine leukemia virus- Transcriptase Reverse). I am expressing in E. coli pTGroE and purifying in a Chelating Sepharose loaded with Ni2+. I am eluting the...

01 June 2020 4,105 4 View

How can you insert measuring items in Projective Techniques?

I am conducting research on consumers' attitudes (namely trust & skepticism) towards Corporate Environmental Responsibility communication and would like to use Projective Techniques (Psychology...

22 March 2020 7,527 3 View

Is it possible to calculate pIC50 of a plant extract?

Is there a way to calculate the pIC50 value of a plant extract? As far as I know, in order to calculate pIC50, the IC50 must need to be in molarity, therefore, it's not possible to get the value...

08 January 2020 8,540 3 View

Why do my protein samples and standards get stuck running Western blot?

When I'm running an 8% SDS-PAGE gel at 100 V, my samples and standards get stuck after a while. Does anyone know why this is happening? Thanks!

20 May 2019 7,797 3 View

Hi, I would like to solubilize a hidrophobic compound in order to administer it to mices. Should anyone suggest a nontoxic vehicle?

The compound is very insoluble in aqueous solution and the compound administration will be given orally. The compound manufacturer has no information regarding the solubility of the compound....

16 January 2019 834 1 View

Anyone can recommend an efficient method for sterilization of corn seeds?

I tried ethanol 70% for 3 min, then bleach 5% for 3 min, followed by 3 rinses of sterile water. Also, tried the same method, but instead of 3 min used 6 min.

10 October 2018 6,640 11 View

Electric Field of Nanowire Transistors

Hello, I am currently working with gate all around nanowire transistors and I have a question regarding the eletric field in the structure. I've developed 4 different structures, made out of...

04 August 2017 4,492 2 View

What is the characteristic length to determine the Reynolds number for the scattered turbulent fluid flow?

Dear all, I just saw this example in FB on the difference between laminar flow and turbulent flow. The flow from a pipe hits the sink and scattered. The flow from the pipe is characterized as...

02 March 2021 1,999 5 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why do the air flow results vary totally when adding scalar in CFD ?

Dear All, We are using Autodesk cfd to calculate the air flow in a single sided naturally ventilated room, where air flow depends only on buoyancy due to temperature difference between inside and...

25 February 2021 9,602 4 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

How to calculate time-averaged fields from Ansys CFX solver?

I would appreciate any comments on the following problem. I need to calculate mean fields (e.g. pressure, velocity and vorticity), for which I activate Trn Stats tab in CFX-Pre. However, what I...

21 February 2021 8,548 7 View

Can we use a compensation matrix with single staining that the negative population for each single staining is below zero?

Recently we used single staining to make a compensation matrix. But, for each single staining we saw that 13 to 25 percent of our negative population had been shown below Zero. In this situation...

21 February 2021 9,982 2 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

Which software is best to analyse multi-phase fluid flows ?

Hello everyone, hope all is good. I want to analyse multi-phase fluid flow through pipe, which software is best to do so ?

21 February 2021 4,230 8 View

Can anyone help with an Accurate Diffuser Expansion Loss formula for rectangular shaped diffusers in turbulent flow regime?

I have tried Idelchik's Handbook of Hydraulic Resistance 4th Edition (2007 page 300) formulas and across some range the formulas are reported valid for, the law of conservation of energy is...

20 February 2021 2,504 1 View

Alexandr Chernov

Hello, Fernanda!

Maybe this instruction will help you:

https://docs.flowjo.com/flowjo/workspaces-and-samples/samples-and-file-types/ws-sampleinfo/

Fernanda Thomazini

Thank you so much Alexandr. I'll see with details this link.

Jitendra Kumar Shandilya

You should ask specific question. What kind of help you need?

Jitendra Kumar Shandilya how to use, even.The basic instructions to analyze samples and interpretation of results.

Attaching link for flowjo tutorial from flowjo University.

Hope this will help you. This is link for 1 chapter of 17, you may browse to all 17 chapters.

Also find attached manual of flow Jo.

https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-in-flowjo/131

All the best.

Suba Nookala

I can do my best to answer. Do you have a specific question?

Jitendra Kumar Shandilya Thank you, I'll see with details soon. Thank you so much

Suba Nookala Thank you Suba, I want o know how to use, even.The basic instructions to analyze samples and interpretation of results. Not too specific yet, the basic.