Software flowjo and flow cytometry. How to use?

More Fernanda Thomazini's questions See All

The Structure Harvester website still not working?

I'm trying to run the Structure Harvester for interpretation based on Evanno method and the website don't work. Someone have any alternatives? Thank you

12 April 2024 2,757 1 View

Does anyone has used/have an opinion about these quantun PAR meter: Apogee DLI-500 and Spectrum SPEC-3415A?

The first costs twice as much as the second in Brazil. Thanks

14 March 2024 9,155 1 View

How can external validation be established for a systematic review / meta-analysis evaluating the impact of a particular gene polymorphism on cancer?

Dear all, We have submitted a manuscript for publication that presents a systematic review and meta-analysis evaluating the influence of a gene polymorphism on cancer risk. The study involved the...

01 February 2024 6,603 4 View

Colony PCR post Gibson Assembly, help?

I did a Gibson assembly reaction and consequently, I did the transformation with my construct to E. coli DH5alpha and I plated the bacteria into 20 µg/mL Kanamycin agar plates. The first time the...

23 January 2024 8,374 3 View

Why Parsifal doesn't import all paper from Mendeley (.bib)?

I manually entered many papers in Mendeley and tried to import them to Parsifal. From 85, 9 are missing in the transfer. I tried to transfer one of the missing ones, and Parsifal informed me the...

17 December 2023 9,248 0 View

Failed Colony PCR?

Hi everyone, I need some help with my colony PCR procedure. Last Week I did a heat shock transformation on E. coli DH5alpha with pUC57 plasmid. I extracted the DNA from the bacterias by heat,...

05 October 2023 3,474 8 View

Are these soil DNA quality good for metagenomic analyses?

I'm planning to conduct Metagenomic analyses on DNA extracted from soil using the ITS and 16S primers. However, I'm getting bands at 2k or 3k on the gel, even though my ratios and DNA...

11 July 2023 8,715 3 View

What is the best brain region to use as a reference for normalization in FDG-PET images where the region of interest is in the somatosensory cortex?

I'm interested in somatosensory metabolism in a model of cortical malformation with susceptibility to absence seizures.

13 June 2023 7,907 1 View

Where do I find the WHO GIS database?

Can anyone tell me where I can find the World Health Organization's regional vectors (shapefile)? There are 6 regions in total: Africa, Americas, Eastern Mediterranean, Europe, Southeast Asia,...

20 April 2023 6,593 0 View

For evaluation of quality evidence in systematic review and meta-analysis in animals, what methods are best or better indicated?

For evaluation of quality evidence in systematic review and meta-analysis in animals, what methods are best or better indicated? GRADE pro, ARRIVE 2.0, or STAIR 2021? To assess the risk of bias, I...

21 February 2023 6,207 3 View

How to determine positive-stained cells in FACS? Use isotype or unstained control?

To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...

06 August 2024 6,728 6 View

Why do we equate male and female arousal?

Women, on the other hand, can become physically aroused (increased blood flow in the reproductive organs) without becoming psychologically aroused even in the slightest. (Robert Weiss)

05 August 2024 9,537 2 View

How to make a Lateral flow control line more uniform?

We have a lateral flow test on Sartorius 140 NC. The conjugate is gold-monoclonal antibody. Using different control lines (GAM, protein A, protein G) we get a very strong leading edge with reduced...

31 July 2024 4,510 3 View

Suggestions for the Annexin V Positive Control ?

Hello everyone, I am currently using washed human platelets to stain Annexin V as a procoagulant marker. Additionally, I am staining with PerCP-CD61 to identify platelet cells. So far, I have...

29 July 2024 8,624 1 View

Why cannot i find my protein on cell surface after antibiotic selection of expressing plasmid?

I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...

28 July 2024 3,178 3 View

Problem with Two-phase simulation?

Hello; I am conducting a CFD study on two-phase flow in a separator, utilizing refrigerant as the working fluid. For this study, I have implemented pressure inlet and pressure outlet boundary...

25 July 2024 2,309 5 View

Flow through curved domains?

Hi everyone, I am working on a curved domain in which a ship is situated in the middle (geometry is given below). In my understanding the general fluid flow is parallel to the x axis from inlet to...

25 July 2024 9,058 4 View

BD Cell Viability Kit - TO evaporation?

Hello, I'm conducting flow cytometry experiments using the BD Cell Viability Kit and I've noticed that the TO solution ends much faster than the PI solution. I store the solutions according to...

22 July 2024 2,257 0 View

Can I use CCCP instead of H₂O₂ as a positive control for ROS measurement through flow cytometry?

I am giving starvation stress to the soil amoeba (Dictyostelium discoideum) cells in my study groups and using NAC as a negative control. I am using DHE for ROS measurement.

20 July 2024 6,354 0 View

Has anyone tried freeze flow samples in -80C freezer or liquid nitrogen before processing?

Our lab has some radioactive cell samples that need to be kept frozen for at least 30 days before flow analysis. For some reason, the samples stored in liquid nitrogen did not perform as well as...

15 July 2024 9,975 1 View

Alexandr Chernov

Hello, Fernanda!

Maybe this instruction will help you:

https://docs.flowjo.com/flowjo/workspaces-and-samples/samples-and-file-types/ws-sampleinfo/

Fernanda Thomazini

Thank you so much Alexandr. I'll see with details this link.

Jitendra Kumar Shandilya

You should ask specific question. What kind of help you need?

Jitendra Kumar Shandilya how to use, even.The basic instructions to analyze samples and interpretation of results.

Attaching link for flowjo tutorial from flowjo University.

Hope this will help you. This is link for 1 chapter of 17, you may browse to all 17 chapters.

Also find attached manual of flow Jo.

https://www.flowjo.com/learn/flowjo-university/flowjo/getting-started-in-flowjo/131

All the best.

Suba Nookala

I can do my best to answer. Do you have a specific question?

Jitendra Kumar Shandilya Thank you, I'll see with details soon. Thank you so much

Suba Nookala Thank you Suba, I want o know how to use, even.The basic instructions to analyze samples and interpretation of results. Not too specific yet, the basic.