Hi
Though, I don't have the exact answer.....but....
In the mouse spleen there are large number of different cell types of varying sizes. And the size varies depending on the culturing protocols and time, how long you are culturing them. In the mean time some cells may be differentiated ... During running flow on disrupted spleen single cell suspension one can get a wide range of size and granularity in a specific spread of FSC vs SSC.
Disrupted spleen single cell suspension (i.e., in whole splenocytes) contains lymphocytes (smaller), RBCs (smaller), granulocytes (larger), monocytes/macrophages (larger), dendritic cells (larger), stromal cells (varying) etc etc etc.
Arun is correct that "splenocytes" is a mixed population of cells. With that said, the vast majority of cells in the spleen (not counting red blood cells) are B-lymphocytes (large majority) and T-lymphocytes (still a lot, but smaller fraction than B-lymphocytes). These cells are very small, and usually have a diameter of approximately 5-10 uM. This is why a cell pellet of 50,000,000 lymphocytes is actually very small when you compare it to other cell pellets of similar cell number.
Hi David,
The size of a spleen is on average 1cm. The size of individual cells is roughly 5-10um as Joseph Cantor mentioned. If you need to get some splenocytes, you can purchase cryopreserved splenocytes from iQ Biosciences ([email protected]). We use these routinely for our in-vitro assays and it will save you some time (processing & prep).
Best Regards,
Omar
5-10 um on average, with resting B and T cells comprising the lower end of the range and macrophages on the upper end. Adult mouse spleens typically contain 1x10e8 white blood cells: 50-60% B cells, 30% T cells, 5% macrophages, and
Hi Joseph, thanks for your post. Do you however have a reference for this or could you possibly be kind enough to direct me to a source
Many Thanks
Hi dear all,
Does anyone know about these immune cell but in the lung?
Thank you in advance for your help
Diana
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