Single step, rapid RNA extraction ?

Shailesh Shiwajirao Sawant @Shailesh_Sawant

12 December 2018 0 10K Report

Any one here tried one step rapid RNA extraction method using EDTA, SDS,2-mercaptoethanol and formamide. I would like to know the results from bacteria.

Badges
Science topic

More Shailesh Shiwajirao Sawant's questions See All

RNA extraction and gel electrophoresis ?

Dear all, I am trying to isolate RNA from bacteria for RNA seq. I have used fresh samples for extraction as well as 3 days stored (-80) samples. After gel electrophoresis I didnt observed 3...

11 December 2018 801 11 View

Selective marker (antibiotic resistance gene)

I am trying to knockout gene in bacterial strain (genome sequence is available) so I must choose the first selective marker but I found that this strain is resistant to aminoglycoside, Blactum...

09 October 2012 1,884 4 View

Quantitative analysis of cellulose in Medium ?

Can anyone suggest me good method for the quantitative determination of cellulose in Broth and medium after fermentation other than DNSA test?

08 September 2012 3,438 4 View

Enzyme Immobilization in polyacrilamide beads

Immobilization of penicillin acylase in porous beads of polyacrylamide gel (...

06 July 2012 3,063 7 View

Separation of nanoparticles according to their shape?

I want to separate nanoparticles according to their shape. I have read that electrophoresis and density gradient centrifugation give good results. Could anyone recommend what the best method for...

06 July 2012 7,974 13 View

Can my bacterial strain synthesize PHA?

I want to check whether or not my isolated bacterial strain is able to synthesize PHA (Polyhydroxyalkanoates). How can we identify PHA content in the bacterial cell by GC chromatography? What is...

05 June 2012 7,854 7 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

E.coli contamination in human RNA seq data ?

Recently, we observed that 99% of the sequences in our RNA-seq data corresponded to the E. coli genome. Despite multiple DNAse treatments after RNA extraction and ribosomal depletion, we were...

06 August 2024 807 3 View

RNA later for the preservation of RNA in fecal samples at room temperature for one day (37°C)?

I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...

06 August 2024 1,367 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to draw the five chelate rings of [Co(EDTA)]- on this picture?

Is this circle one of the rings?

03 August 2024 5,988 0 View

PBMC infection with virus?

In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...

01 August 2024 4,403 4 View

What is the acceptable p-value cutoff for GO enrichment analysis ?

I have an RNA-seq data that I have analysed using Limma-voom and have extracted the gene IDs, log2FC and the p-values. At p value < 0.05, I have over 10,000 DEGs, however, when I run the GO...

31 July 2024 225 2 View