7 Questions 25 Answers 0 Followers
Questions related from Shailesh Shiwajirao Sawant
Any one here tried one step rapid RNA extraction method using EDTA, SDS,2-mercaptoethanol and formamide. I would like to know the results from bacteria.
12 December 2018 9,765 0 View
Dear all, I am trying to isolate RNA from bacteria for RNA seq. I have used fresh samples for extraction as well as 3 days stored (-80) samples. After gel electrophoresis I didnt observed 3...
12 December 2018 776 11 View
I am trying to knockout gene in bacterial strain (genome sequence is available) so I must choose the first selective marker but I found that this strain is resistant to aminoglycoside, Blactum...
10 October 2012 1,856 4 View
Can anyone suggest me good method for the quantitative determination of cellulose in Broth and medium after fermentation other than DNSA test?
09 September 2012 3,410 4 View
I want to separate nanoparticles according to their shape. I have read that electrophoresis and density gradient centrifugation give good results. Could anyone recommend what the best method for...
07 July 2012 7,951 13 View
Immobilization of penicillin acylase in porous beads of polyacrylamide gel (...
07 July 2012 2,993 7 View
I want to check whether or not my isolated bacterial strain is able to synthesize PHA (Polyhydroxyalkanoates). How can we identify PHA content in the bacterial cell by GC chromatography? What is...
06 June 2012 7,796 7 View
