Single step, rapid RNA extraction ?

Any one here tried one step rapid RNA extraction method using EDTA, SDS,2-mercaptoethanol and formamide. I would like to know the results from bacteria.

11 December 2018 9,684 0 View

RNA extraction and gel electrophoresis ?

Dear all, I am trying to isolate RNA from bacteria for RNA seq. I have used fresh samples for extraction as well as 3 days stored (-80) samples. After gel electrophoresis I didnt observed 3...

11 December 2018 704 11 View

Selective marker (antibiotic resistance gene)

I am trying to knockout gene in bacterial strain (genome sequence is available) so I must choose the first selective marker but I found that this strain is resistant to aminoglycoside, Blactum...

09 October 2012 1,776 4 View

Enzyme Immobilization in polyacrilamide beads

Immobilization of penicillin acylase in porous beads of polyacrylamide gel (...

06 July 2012 2,917 7 View

Separation of nanoparticles according to their shape?

I want to separate nanoparticles according to their shape. I have read that electrophoresis and density gradient centrifugation give good results. Could anyone recommend what the best method for...

06 July 2012 7,879 13 View

Can my bacterial strain synthesize PHA?

I want to check whether or not my isolated bacterial strain is able to synthesize PHA (Polyhydroxyalkanoates). How can we identify PHA content in the bacterial cell by GC chromatography? What is...

05 June 2012 7,720 7 View

No title

02 March 2021 3,060 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

How was your experience with Biomedical and Biotechnology Journal?

How was your publication experience with BBRJ in terms of peer review and reviewer comments. Did you find it fair and transparent ?

23 February 2021 3,810 6 View

Are the topics below viable research in plant biotechnology?

1. Chemically induced mutagenesis in seed and qPCR detection and amplification of desired trait. 2. Site-directed mutagenesis in qualitative traits of a multiline seed.

04 February 2021 9,069 3 View

Is receiving Invitation to submit a article from the journal is big deal ???

I have recently received a invitation from the journal "Current Pharmaceutical Bio-technology" to submit a research article. As i didn't received any invitation before. so, is it normal to to...

04 February 2021 4,596 23 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

What steps should one take to address post-harvest disease problems in a supply chain?

If I were to receive an import of a new fruit species in the country and, upon arrival, the whole lot was diseased, what steps do I take to diagnose and solve the problem? Is there a general...

10 January 2021 1,649 7 View

Why negative controls also showing the specific bands of promotor and other regions (GUS, GFP etc.)?

Dear Researchers, I did a transformation of an empty vector (pCAMBIA1301/1302) into my passion fruit plants, for the optimization of the transformation protocol. After transformation; DNA...

06 January 2021 8,465 99 View