Whether it possible to quantify Ciprofloxacin and Gentamicin simultaneous by HPLC method or not. Please suggest other alternate method to quantified both in the same sample.
Hi
Many publications describe the methods for example:
Article A liquid chromatography-tandem mass spectrometry platform fo...
...the key question is about available detection systems that could be very helpful for example MS/MS ....
Best regards
Tomasz
I just checked and there are many publications on this specific analysis that you can use. Still, you may need to change some factors on your own instrument to make sure you can separate the peaks as much as possible, prevent overlapping and get the best shape of peaks. In case non of these worked out for you, then you may need to try chemometrics methods like PLS to do the silmultanues analysis. This may be helpful for you to get an idea what I'm talking about: https://pubs.rsc.org/en/content/articlelanding/2016/ra/c6ra18415d#!divAbstract
Hi Awadh.
The challenge is separate all Gentamicin components and Cyprofloxacin in this method. One alternative is use mass detector like Tomasz Grabowski said or try separate Gentamicin components and Cyprofloxacin in HPLC.
If you are able to derivate the gentamicin with hydrophobic and chromophore carrying agents then it is possible to separate and detect both compounds using reversed-phase conditions and UV detection. Regarding the detection possibility without modifying the molecules, it is possible only by implementing the ELSD, CAD, MS, ECD detectors to the HPLC system. On the other hand, there is not available any column chemistry that can give good chromatography with reasonable retention for quantification at the simultaneous analysis of these compounds due to completely opposite polarities of the molecules.
I wish to know whether it possible to use PDA detector to identify both component in the same sample. Last option to use LCMS to identify both component in the same samle. Thank you everyone for your promot response which help me to decide which one better method available to detect and wuantify both drug in the same sample.
PDA is never a choice for identification. It provides excellent data for quantification but you cannot use it for absolute identification; you would need to move to a MS or IR type detector that can provide unequivocal spectral data. Once you have your method then you can port it to the PDA if your sample is free of interferences.
analysis is the simultaneous determination of two drugs without prior separation. HPLC method and spectrophotometric methods are available for simultaneous determination of ciprofloxacin and gentamycine, so there was a need to develop an accurate method for their determination
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