Please suggest the statistical tests to find which changes in habitat features have influenced the change in species abundance?

I have two datasets of abundance count (continuous response variable) and habitat features (continuous explanatory variable). One was collected in the year 2000 and another collected in the year...

03 July 2020 5,185 6 View

Can anybody help me with the troubleshooting of trichrome staining of rat uterus?

I am using the standard protocol as given in the Bancroft's book,the color distinction is fine but getting pink blotches all over my tissue section..Have increased washing time in each step and...

22 January 2020 1,471 3 View

Can any one please provide me a protocol for how to extract and purify alpha amylase from animal pancreas?

I want to extract alpha amylase from goat pancreas. And alpha amylase and alpha glucosidase are same enzymes?

01 May 2019 6,151 3 View

Why male Wistar rats are preferred over females in diabetes research?

I am a research scholar , conducting research on antidiabetic potential of herbal compounds. I have found that in vivo testing of anti diabetic drugs are mostly done on male wistar rats. So,...

01 November 2018 2,199 6 View

How do I model a rope which can take only tension made of 3D solid elements?

I have tried the following and it doesn't work overlaying the two material properties and assigning the corresponding section properties to rope with different modulus in compression(1MPa)(very...

07 January 2018 8,334 9 View

How to compare avian diversity of two different water bodies?

30 April 2017 9,418 8 View

How I identify the different intensity zone of a cell by MATLAB contrast thresholding?

I take a picture by microscope in which cells are present in different intensity zone. How I separate or segment them? For a particlur threshold it will show particular cell, changing the...

13 September 2016 3,282 4 View

Any suggestion to remove ammonium formate from the preparative elution so that I can get impurity in its pure form?

I have isolated an unknown impurity using Preparative HPLC and mobile phase 0.02 M Ammonium Formate in water. I have already tried extractions with DCM & Ethyl Acetate.

16 March 2015 9,055 3 View

How do I prepare the stock solution when using SMAC Mimetics (5-Aminoindole)?

24 September 2014 5,803 1 View

Does anyone know the method of finding a cause effect relationship with 4 independent factors?

The factors have been derived from a single questionnaire; one possibility is regression method. I wish to know if there are any other possibilities.

02 April 2014 9,600 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How two melting peaks in DSC of polypropylene can be merged into one crystallization peak?

The figure shows DSC cooling (blue) and 2nd heating (green) curves (step: 10 K/min) for a commercial sample supplied as Atactic Polypropylene (APP). The heating curve illustrates two major...

03 March 2021 5,857 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

How to compare different concentrations of nanoparticles for characterization from ICP-MS?

I took measurements of different concentrations of 15 nm and 5 nm Au nano particle solutions. I'm trying to determine which concentration I measured gives the best reading overall. I also...

02 March 2021 7,626 1 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View