Please suggest the statistical tests to find which changes in habitat features have influenced the change in species abundance?

I have two datasets of abundance count (continuous response variable) and habitat features (continuous explanatory variable). One was collected in the year 2000 and another collected in the year...

03 July 2020 5,185 6 View

Similar FPLC peaks for two different samples. How to proceed for sequence identification?

I ran fplc for ultrafiltered samples of muscle and viscera from a fish. Both peaks were extremely similar. Is it required that this denotes the same protein in both samples? Could someone advise...

14 November 2019 980 3 View

Can any one please provide me a protocol for how to extract and purify alpha amylase from animal pancreas?

I want to extract alpha amylase from goat pancreas. And alpha amylase and alpha glucosidase are same enzymes?

01 May 2019 6,151 3 View

Why male Wistar rats are preferred over females in diabetes research?

I am a research scholar , conducting research on antidiabetic potential of herbal compounds. I have found that in vivo testing of anti diabetic drugs are mostly done on male wistar rats. So,...

01 November 2018 2,199 6 View

How do I model a rope which can take only tension made of 3D solid elements?

I have tried the following and it doesn't work overlaying the two material properties and assigning the corresponding section properties to rope with different modulus in compression(1MPa)(very...

07 January 2018 8,334 9 View

How to compare avian diversity of two different water bodies?

30 April 2017 9,418 8 View

How I identify the different intensity zone of a cell by MATLAB contrast thresholding?

I take a picture by microscope in which cells are present in different intensity zone. How I separate or segment them? For a particlur threshold it will show particular cell, changing the...

13 September 2016 3,282 4 View

Any suggestion to remove ammonium formate from the preparative elution so that I can get impurity in its pure form?

I have isolated an unknown impurity using Preparative HPLC and mobile phase 0.02 M Ammonium Formate in water. I have already tried extractions with DCM & Ethyl Acetate.

16 March 2015 9,055 3 View

How do I prepare the stock solution when using SMAC Mimetics (5-Aminoindole)?

24 September 2014 5,803 1 View

Does anyone know the method of finding a cause effect relationship with 4 independent factors?

The factors have been derived from a single questionnaire; one possibility is regression method. I wish to know if there are any other possibilities.

02 April 2014 9,600 2 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

Does anyone know how to plot my station to a section plot on Ocean Data View (ODV)?

I made a vertical section plot with WOA .nc file on ODV, and now I want to plot my stations (csv. file) in it. Does anyone know how to import my points to the section? They are two different...

01 March 2021 3,610 1 View

Does anyone know an alternative product for cardiac Troponin T antibody for Immunofluorescence (Thermo # MA512960)?

We have been using Thermo Fisher anti-cardiac Troponin T antibody for immunofluorescence staining in FFPE mouse heart sections but it was recently discontinued. The literature shows it to be the...

01 March 2021 4,937 1 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View

Any specific pipeline or parameter to analyse total transcriptomics study of brain tissue samples?

I have two groups of brain samples, control and treated for example. It was total RNA nova seq sequencing. I tried all the available pipeline like: star+rsem+deseq2, Hista+stringtie+cuffdiff,...

27 February 2021 356 6 View

B-mercaptoethanol (BME) protocol?

Hi everybody, I have been running a multiplex IHC which allow me to stain the same tissue with different markers (Primary Ab + Secondary conjugated Ab) (AF487, AF555, AF647). Since I would like to...

27 February 2021 5,028 2 View

Why do my MCFs not form monolayer?

Hello, I am looking for some expertise on culturing MCF7s. I purchased them from ATCC and I have been following all media and culture conditions. Media is EMEM with 10% FBS and 10ug/mL of...

27 February 2021 3,904 2 View

Is there anyone who experienced with cytotoxic effect of Hoescht 33342 dye?

Is Hoechst toxic to cells? In general, it is known as non-toxic. I am working with dsRED MDA-MB-231 cell line. I applied it for my PDMS-based microfluidic platform, for this reason, I incubated...

26 February 2021 9,119 3 View