I tried to synthesize silver nanocluster and tested it with different concentration of DNA, AgNO3 and NaBH4, but we observe a black precipitation without any flourescent and absorbance. Is there anyone with this experience to guide me?
The reduction of silver ions to silver occurs in stages. Ions>atoms> clusters> nanoparticles> silver phase. At each stage the object must be stabilized. For this, except for the phase. it is necessary to use a stabilizer. If you do not get nanoparticles, then you have poorly stabilized the dispersed system.