I am using glass milk (silica) to purify RNA. However the protocol I am following uses acidic KI. Does the chaotropic agent has to be acid to allow RNA binding to the silica?
Dear Monica, thank you for posting this very interesting technical question on RG. As an inorganic chemist I'm unfortunately not a specialist in this field. However, I would like to suggest to you the following closely related RG threads which might give you some explanation:
What happens to DNA in the presence of a chaotropic salt?
https://www.researchgate.net/post/What-happens-to-DNA-in-the-presence-of-a-chaotropic-salt
(12 answers)
and
What is the use of chaotropic agents in spin column-based nucleic acid purification?
https://www.researchgate.net/post/What_is_the_use_of_chaotropic_agents_in_spin_column-based_nucleic_acid_purification
(6 answers)
Also please have a look at the following potentially useful article:
Adsorption and elution characteristics of nucleic acids on silica surfaces and their use in designing a miniaturized purification unit
Article Adsorption and elution characteristics of nucleic acids on s...
Good luck with your work and best wishes!
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