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I am using glass milk (silica) to purify RNA. However the protocol I am following uses acidic KI. Does the chaotropic agent has to be acid to allow RNA binding to the silica?
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I am looking for an easy protocol to purify total lipids from yeast as I have several strains and conditions.
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When measuring the relationship between budded and unbudded yeast we do not know if we should include cells with large buds, 75% of the size of the mother cell, as budded or unbudded.
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