Should I use same buffer (low pH) as refolding buffer pH for protein purification?

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Hassan Nima

this link may be useful

alfresco.ubm-us.net/alfresco_images/pharma/.../article-87987.pdf

regards

Omar Gonzalez-Ortega

You can but it is not appropriate. pH has to be at least 7 for imac thus you need to use another buffering molecule. I do not understand the refolding part, could you elaborate in the whole process you are running.

Dominique Liger

Hi there,

Ideally working pH should be at least 1 unit away from pI. For IMAC, imidazole of histidines has to be deprotonated therefore working pH should be at least 7.

Sadaf Sulman

I used refolding kit to find the best refolding buffer for my protein as its in inclusion bodies.

Thank you all for your response!