Sadaf Sulman

2 Questions 4 Answers 0 Followers

Questions related from Sadaf Sulman

Should I use same buffer (low pH) as refolding buffer pH for protein purification?

The refolding buffer (50mM MES) I tried for my protein has pH 6.0, pI of protein is also 6.2. Next step is I want to purify it through Ni-NTA resin. Should I maintain same buffer MES and pH 6.0...

04 April 2018 4,214 4 View

My his-tag protein has low expression level and it doesn't bind NiNTA column and elute in the flow through. Why?

pI above 9

03 March 2017 545 4 View