I have some count data from kallisto. I have six replicates, but i am ask to say what is the most highly expressed RNA in a specific one of these replicates
Hello multivariate gurus, I have a question regarding RDA multivariate analysis. Typically this is used to explain variation in a species/abundance dataset using envinonmental variables as a...
30 March 2023 5,588 5 View
I am using Nanopore to sequence 16S/23S in a microbiome study. I am about to start looking at my sequencing data (removing adapters, trimming data) and found myself unsure whether both strands of...
25 August 2022 9,925 1 View
Dear fellow researchers, I wish to do a moderated serial mediation in PROCESS. Hayes book (version 2013) shows a short overview of different models. However, moderated serial mediation models are...
14 June 2022 6,780 3 View
I added 3 µg plasmid DNA containing the GFP gene to 75 µL optimem and I added 7,5 µL lipofectamine 2000 reagent to 75 µL optimem. I've incubated the plasmid DNA with lipofectamine and optimem at...
23 September 2021 5,249 3 View
I'd like to characterize an unknown bacteria strain which was stored in a glycerol stock at -80 degrees Celcius. I've inoculated the unknown bacteria strain on an LB agar plate. The colonies of...
14 June 2021 7,865 4 View
Hello, I am looking to obtain the following cell lines for an experiment: NT-3 1.1B4 TRM-1 Blox5 CM PAN-IN Would anyone know anyone who are using these cell lines that would be able to...
01 June 2020 9,965 0 View
Hi everyone, I would need some help with calculating the area under the curve. In my study patients' heart rate was measured at different time points throughout an operation (at the start of the...
29 November 2019 1,147 4 View
Hello, I have performed a TAG assay on adult male and female Drosophila, however, I seem to end up with other values that that are reported in literature (40-50µg per mg fly). My relative levels...
04 July 2019 1,358 2 View
For and experiment I would like to place Drosophila on standard food suppelemented with 20% coconutoil. As described in other papers, the vials can be kept at 25°C. Unfortunatly my flies die due...
25 March 2018 3,860 3 View
In NOD SCID it was shown that function of APC cells is reduced due to the NOD background. Does that mean that in the RAG2-/- gC-/- (only T- B- and NK-cells are absent) and macrophages are still...
05 November 2016 9,298 2 View
I am trying to simulate vehicular loading on an orthotopic steel deck bridge section in ABAQUS software. The red arrow mark in the attached figure indicates the direction in which the vehicle will...
08 August 2024 719 0 View
Hi, we have measured tryptic peptides using both DDA and DIA method on QExactive. In DDA replicates i saw unusual intensity drops occurring at the same sections of chromatograms in DDA replicates...
07 August 2024 3,218 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
I am trying to put 3 separate plasmids into Staphylococcus aureus and I am looking for those that are theta replicating and compatible. I have obtained pSK9065 (from pSK1) from Addgene, but the...
23 July 2024 6,968 0 View
I am trying to replicate results published in the journal paper published recently (Modeling of Laser-assisted micro-milling Inconel718 ). In that paper, they used vdflux subroutine to obtain...
05 July 2024 3,701 0 View
Hi anyone, I have got in to trouble, We have pet plasmids type 28 and 21 and I have found pet plasmids can't replicate in to pseudomonas, can anyone tell that why pet plasmids, especially, 28 and...
25 June 2024 7,083 1 View
Hello. We have a sample with an unknown concentration of polyphenols. We're diluting the sample in order to perform the Folin Ciocalteau method. 0.2 grams sample / 4 mL 70% methanol and 30%...
10 June 2024 2,134 3 View
Hi all, Hoping for some troubleshooting/advice. Currently setting up a virus typing assay for routine use. I have the assay working with good sensitivity (regularly amplifies samples that are...
09 June 2024 4,468 3 View
I want to replicate the third step, but there seems to be no reaction in an alkaline environment. However, sodium hydroxide is used in the literature. Should I switch to an acidic environment
05 June 2024 9,358 1 View
I am doing a RT-qPCR for gene expression analysis of cancer patients, and there are four different groups in the results. The first group that shows over expression of my target gene (both two...
31 May 2024 1,088 0 View