Hi everyone,
I've been having issues on trying to define whether I am going to use a specific thresholding for my image analysis or should I perform automatic thresholding on Image J?
I am analyzing alveolar bone healing after tooth extraction with different photobiomodulation protocols. I runned microcomputed tomography in all my samples and now I am processing them on ImageJ and BoneJ.
However, I thought I should specify the threshold (120-255, for example) and apply the same to every image. But I've seen contrasting studies. A lot of them perform automatic thresholding.
This has concerned me since the results of the analysis change a lot when I perform one or another.
Does anyone have experience on that?
Apppreciate every contribution.
Hi,
Though I have no experience in microCT imaging, I can suggest some troubleshoot from imageJ prospective. You are probably having this issue because your image's foreground is not clearly distinguished from background and hence, imageJ is wrongly guessing the edges of ROI. Try matching your image (brightness of region of interest and darkness of background) with the studies images you refered. If you cannot edit(brightness/contrast) or take images again of the samples. I suggest you should predetermine the thresholding value so you only calculate the ROI.
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