How to graph non expression in some of the treatments in RT-qPCR?

Hello everyone. I have an experiment of 5 treatments (including the control) and have checked several genes of interest. Some of these genes were expressed in some treatments but not others. When...

28 December 2020 8,418 4 View

Which is the correct way to analyze RT-qPCR data in paired and unpaired samples?

I have read the relevant paper from Schmittgen and Livak (2008) to explore the examples. I have also extensively read and combined all the threads on Research Gate involving answers about RT-qPCR....

30 November 2020 6,305 9 View

How do i calculate equivalent von Mises stress in a 2d fem model?

I want to calculate the equivalent stress for an elasto-plastic analysis.Which is the correct way to do it: 1) Calculate stresses at each Gauss Point and then evaluate equivalent stress at each...

30 September 2020 2,379 3 View

Stable gene silencing without viral vctors??

I am looking to create stable cell lines HCT116 for gene silencing . I am not satisfied with the siRNA experiments ( not great transfection efficiency so far and

28 February 2020 4,063 3 View

Hi there, I'm currently searching for a 2D or a 3D coastal cliff erosion model. Any suggestions?

There's quite a few models on coastal beach or dune evolution, however neither of them include coastal cliff erosion modules.

05 February 2019 709 4 View

Can higher order Lagrange shape functions increase the continuity inside the element?

My question is can we have higher order continuity inside an element while it has C0 continuity between other elements?For example a plane element has C0 continuity between elements can we...

30 January 2019 1,553 3 View

How do I know if my stem cells are still pluripotent?

12 October 2014 1,868 12 View

How is it possible to discriminate if a colloidal solution contains metal or metal oxide nanoparticles?

Which characterization technique is the most suitable?

15 September 2014 164 6 View

Can we use 3D elements for bending problems?

Can we use solid elements for bending problems?If yes do these elements give accurate results?

01 January 1970 3,030 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS?

I need to model masonry and reinforced concrete minarets for my MSc thesis. Which one is easier and better at modeling slender masonry structures? ANSYS or ABAQUS? (Or any other program for just...

01 March 2021 736 3 View

How to assign different material properties to each element in Abaqus ?

Hello, I am writing a python script to generate my model and write the input file. The problem is I have 1000 elements in my mesh and I want to assign different material properties to each...

01 March 2021 9,511 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

For modelling epoxy-based matrices (in composites), which model in ABAQUS is the most suitable, Concrete damaged plasticity or Drucker-Prager model?

I am working on developing a micromechanical FE model for predicting kink-band formation in UD composites. To model matrix plasticity, which model, out of Concrete Damaged Plasticity and...

28 February 2021 5,415 1 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials?

Hi, all! How can we use a hybrid element in Abaqus Dynamic/Explicit for impressible materials? I have not find the choice for explicit element in Abaqus CAE. I am wondering whether it is possible...

25 February 2021 9,936 2 View

How to measure angle between laser travel path and machine axis in Laser Measurement system?

25 February 2021 1,987 3 View