How much is the proper salinity in ppm for egg water in zebrafish?

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Is it possible to have higher TMRE fluoresence intensity signal in H2O2 treated cells rather than cells treated with H2O2+antioxidants?

I treated SK-N-SH cells with 1mM H2O2 and 1mM H2O2+antioxidants (3 different mixtures) and measured the mitochondrial membrane potential (ΔΨm) with 500nM TMRE. As far as I know, H2O2 and ROS...

18 October 2021 2,706 14 View

How to graph non expression in some of the treatments in RT-qPCR?

Hello everyone. I have an experiment of 5 treatments (including the control) and have checked several genes of interest. Some of these genes were expressed in some treatments but not others. When...

28 December 2020 8,576 4 View

Which is the correct way to analyze RT-qPCR data in paired and unpaired samples?

I have read the relevant paper from Schmittgen and Livak (2008) to explore the examples. I have also extensively read and combined all the threads on Research Gate involving answers about RT-qPCR....

30 November 2020 6,488 9 View

How do i calculate equivalent von Mises stress in a 2d fem model?

I want to calculate the equivalent stress for an elasto-plastic analysis.Which is the correct way to do it: 1) Calculate stresses at each Gauss Point and then evaluate equivalent stress at each...

30 September 2020 2,519 3 View

Stable gene silencing without viral vctors??

I am looking to create stable cell lines HCT116 for gene silencing . I am not satisfied with the siRNA experiments ( not great transfection efficiency so far and

28 February 2020 4,202 3 View

Regeneration of phosphorus in microalgae batch bultures?

Is it possible to notice an increase in phosphorus concentration in batch cultures of microalgae strains after several days of normal decrease of nutrients?

11 June 2019 5,146 3 View

Hi there, I'm currently searching for a 2D or a 3D coastal cliff erosion model. Any suggestions?

There's quite a few models on coastal beach or dune evolution, however neither of them include coastal cliff erosion modules.

05 February 2019 856 4 View

Can higher order Lagrange shape functions increase the continuity inside the element?

My question is can we have higher order continuity inside an element while it has C0 continuity between other elements?For example a plane element has C0 continuity between elements can we...

30 January 2019 1,676 3 View

Does C-1 continuity of the stress field leads to not accurate approximation of it?

In linear fem for truss and plane elements we have C-1 continuity regarding stresses.Does that lead to a worse accuracy or approximation than C1 continuity?

23 January 2019 2,432 0 View

Which has better accuracy 6-node triangular element or 4-node quadraqular element?

The 6node triangular element and the 4node quadrangular have the same order (p=2), so which one has better accuracy and why?

18 January 2019 3,565 7 View

How to quantify polystyrene microplastic (8 micron) bioaccumulation in fish tissue?

"I have treated adult zebrafish with 8-micron polystyrene microplastic and want to study the bioaccumulation in different organs. Can this be done using hydrogen peroxide digestion followed by...

05 August 2024 853 3 View

In passive sampling, can polymer-lipid partition coefficients be applied independent of environmental salinity?

For example, if low-density polyethylene (LDPE) is equilibrated with PCB contaminated marine and freshwater sediments, can LDPE-lipid partition coefficients be used to obtain equilibrium...

28 July 2024 263 1 View

How to convert water conductivity in mS/cm to Salinity and TDS?

In order to test different water parameters I need to know how to convert conductivity to Salinity and TDS in ppm or ppt. Give me the reference please.

27 July 2024 2,414 2 View

Could GSH level be more in CCL4 rats than negative control rats?

i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....

26 July 2024 1,887 0 View

What methods can be used to minimize environmental variability in breeding trial ?

In breeding trials, environmental variability can significantly affect the results, making it challenging to accurately evaluate the performance of different lines and hybrids.

22 July 2024 3,961 4 View

Can anyone recommend a brand of cas9 protein that can be used for in-vivo editing in model animals?

zebrafish and mouse

19 July 2024 9,158 1 View

How can i generate a CRISPR knockin mutation zebrafish model with a reporter?

Hey! I aim to generate a transgenic knockin zebrafish line that mimetizes a genetic condtition that leads to a certain disease on human. To do so, I need to insert a codon for mutagenic aminoacid...

14 July 2024 6,240 0 View

Does egg white interfere with any histology staining?

I have mouse lung tissue which is mounted with egg whites.I need to stain my tissues with PAS and HE. Can egg whites interfere with these stainings?

02 July 2024 8,261 0 View

Can someone explain the pros and cons of mean squares vs. Baker's ratio for pinpointing gene action in line x tester designs?

In plant breeding research, line x tester mating designs are often used to assess gene action for various traits. Some studies suggest that the simultaneous significant mean squares for lines,...

30 June 2024 4,877 2 View

How can ongoing education and team-basedsimulations improve the effective use of normalsaline in clinical practice?

Overview of Normal Saline in Fluid Therapy: Mechanisms, and ... How can ongoing education and team-basedsimulations improve the effective use of normalsaline in clinical practice?

27 June 2024 3,972 1 View