How to graph non expression in some of the treatments in RT-qPCR?

Hello everyone. I have an experiment of 5 treatments (including the control) and have checked several genes of interest. Some of these genes were expressed in some treatments but not others. When...

28 December 2020 8,418 4 View

Which is the correct way to analyze RT-qPCR data in paired and unpaired samples?

I have read the relevant paper from Schmittgen and Livak (2008) to explore the examples. I have also extensively read and combined all the threads on Research Gate involving answers about RT-qPCR....

30 November 2020 6,305 9 View

How do i calculate equivalent von Mises stress in a 2d fem model?

I want to calculate the equivalent stress for an elasto-plastic analysis.Which is the correct way to do it: 1) Calculate stresses at each Gauss Point and then evaluate equivalent stress at each...

30 September 2020 2,379 3 View

Stable gene silencing without viral vctors??

I am looking to create stable cell lines HCT116 for gene silencing . I am not satisfied with the siRNA experiments ( not great transfection efficiency so far and

28 February 2020 4,063 3 View

Can higher order Lagrange shape functions increase the continuity inside the element?

My question is can we have higher order continuity inside an element while it has C0 continuity between other elements?For example a plane element has C0 continuity between elements can we...

30 January 2019 1,553 3 View

How do I know if my stem cells are still pluripotent?

12 October 2014 1,868 12 View

How is it possible to discriminate if a colloidal solution contains metal or metal oxide nanoparticles?

Which characterization technique is the most suitable?

15 September 2014 164 6 View

Can we use 3D elements for bending problems?

Can we use solid elements for bending problems?If yes do these elements give accurate results?

01 January 1970 3,030 2 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

After a reference from Int J Body Compos Res, can any body help?

Hi, I am after the reference below, my library says it cannot obtain a copy either locally or internationally, any help appreciated! Chris Wang ZM, Heshka S, Wielopolski L, Pi-Sunyer FX, Pierson...

03 March 2021 6,193 1 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View

Shall I skip the measurement model in SmartPLS?

Hello everyone, I had run a mediation model in SmartPLS using questionnaires that were already validated among my statistical population. A professor who edited my paper -though she is not...

01 March 2021 3,052 3 View

What is involved in morphological changes of SEM images of a substance?

In the sem analysis of CaCO3 nanaoparticles, the morphology images showed spherical shaped particles but the proposed morphology for these type of nanoparticles is trigonal. I prepared the...

01 March 2021 1,960 4 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to define tie constraint between rigid body and another part in dynamic explicit model of Abaqus?

Hi dear researchers I connected a rigid body into the part of my structure by tie constraint and then I added displacement to rigid body, in static general step every thing is fine and these two...

28 February 2021 3,442 2 View

Does the circuit modeling for planar monopole antennas using Foster canonical forms give good approximation?

Hi researchers, I am searching for some accurate way to do the circuit modeling of planar CPW-fed monopole antennas. I have gone through the literature where mostly circuit modeling is done using...

28 February 2021 7,016 1 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View