How to graph non expression in some of the treatments in RT-qPCR?

Hello everyone. I have an experiment of 5 treatments (including the control) and have checked several genes of interest. Some of these genes were expressed in some treatments but not others. When...

28 December 2020 8,418 4 View

Which is the correct way to analyze RT-qPCR data in paired and unpaired samples?

I have read the relevant paper from Schmittgen and Livak (2008) to explore the examples. I have also extensively read and combined all the threads on Research Gate involving answers about RT-qPCR....

30 November 2020 6,305 9 View

How do i calculate equivalent von Mises stress in a 2d fem model?

I want to calculate the equivalent stress for an elasto-plastic analysis.Which is the correct way to do it: 1) Calculate stresses at each Gauss Point and then evaluate equivalent stress at each...

30 September 2020 2,379 3 View

Stable gene silencing without viral vctors??

I am looking to create stable cell lines HCT116 for gene silencing . I am not satisfied with the siRNA experiments ( not great transfection efficiency so far and

28 February 2020 4,063 3 View

Hi there, I'm currently searching for a 2D or a 3D coastal cliff erosion model. Any suggestions?

There's quite a few models on coastal beach or dune evolution, however neither of them include coastal cliff erosion modules.

05 February 2019 709 4 View

Can higher order Lagrange shape functions increase the continuity inside the element?

My question is can we have higher order continuity inside an element while it has C0 continuity between other elements?For example a plane element has C0 continuity between elements can we...

30 January 2019 1,553 3 View

How do I know if my stem cells are still pluripotent?

12 October 2014 1,868 12 View

How is it possible to discriminate if a colloidal solution contains metal or metal oxide nanoparticles?

Which characterization technique is the most suitable?

15 September 2014 164 6 View

Can we use 3D elements for bending problems?

Can we use solid elements for bending problems?If yes do these elements give accurate results?

01 January 1970 3,030 2 View

How to define and get results (stresses) for certain points of a model in abaqus, using python? When the size of the model changes?

I want to do a parameter study of a aixsymmetric cylinder in Abaqus. I want to create several jobs where I get the stresses for 3 different points in my model. These points are defined. I wrote a...

01 March 2021 10,078 1 View

LOAD DISPLACEMENT CURVES Stiffness increasing in ANSYS non linear analysis. HOW CAN I CORRECT IT?

Hi. I am doing non linear FE analysis using a new material beam under flexural 2 point loading using ANSYS. I have defined a bilinear material model. The solution has converged. In results I am...

01 March 2021 9,911 3 View

Joint behaviour in Abaqus is not captured well?

I am working on roof joint and trying to simulate the behaviour in Abaqus. I am successful in running the model but the final graph does not follow the experimental curve in the negative direction...

01 March 2021 6,661 3 View

How to modify Johnson cook equation in ABAQUS?

I wanted to add an extra parameter to the existing Johnson cook equation in the ABAQUS simulation. How to add those extra parameters. Ref: A modified Johnson-Cook material model with strain...

28 February 2021 4,841 3 View

How can we simulate an hyperelastic-viscoelastic behavor with Ansys APDL?

I am working on modeling and simulation of biomecanical material behaviour, I have succeded on simulating skin using anisotropic hyperelastic material, on APDL and in our lab's finite element...

28 February 2021 552 3 View

How to find the right number of integration points in second order 3d finite elements?

I am familiar with finite element simulations and numerical schemes like the Gauss Legendre quadrature. I also know that the Gauss integration yields exact values for polynomials up to degree 2n-1...

27 February 2021 6,818 3 View

What could be the reason for not having further low chi square value (better agreement) in my refinement result?

I have tried every combination of parameter changes and after that i have a poor chi square value. I can't get it lower any further. I know there have been a lot of discussion about Rietveld...

26 February 2021 8,480 3 View

How relevant is Mini slump cone?

for all the rheology researchers, my question is, how relevant is a mini slump cone (smaller than a standard 50 cent rheometer). currently based on testing, the thickness of slumped material is...

26 February 2021 3,668 1 View

What is the best way to calculate the angles of twist for concrete columns subjected to torsional moment using finite element software?

In Finite element software like ANSYS , there is difficulty in obtaining direct results of twisting of concrete members because SOLID65 which is the unique element designed to represent concrete...

26 February 2021 3,806 2 View

Stress/strain tensors decomposition ???

25 February 2021 6,760 1 View