29 December 2017 2 8K Report

I am new to IHC. I looked over several protocols for IHC of tyrosine hydroxylase (TH) and saw that some use 1XPBS, some use 0.1M PBS (10X), or PBS+Triton X-100, or TBS, for rinsing sections and diluting antibodies. I am not sure it is simply personal preference or is there benefit to using a particular over over the others. Could someone please tell me which one I should use? Thanks in advance.

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