Should I use 0.1M PBS or 0.01M PBS (1xPBS) for IHC washing and incubation steps?

I am new to IHC. I looked over several protocols for IHC of tyrosine hydroxylase (TH) and saw that some use 1XPBS, some use 0.1M PBS (10X), or PBS+Triton X-100, or TBS, for rinsing sections and...

29 December 2017 8,362 2 View