Ying Wang

2 Questions 18 Answers 0 Followers

Questions related from Ying Wang

Are dehydration steps necessary for DAB-based IHC of mouse brain sections?

My plan is to do free-floating sections for IHC of tyrosine hydroxylase. My questions are: 1) should I dehydrate the sections before mounting them onto slides? 2) I think I need I do a...

12 March 2018 1,405 3 View

Should I use 0.1M PBS or 0.01M PBS (1xPBS) for IHC washing and incubation steps?

I am new to IHC. I looked over several protocols for IHC of tyrosine hydroxylase (TH) and saw that some use 1XPBS, some use 0.1M PBS (10X), or PBS+Triton X-100, or TBS, for rinsing sections and...

29 December 2017 8,437 2 View