Should I expect (Cre dependent) Cas9 expression in myeloid cell culture from mice with the LyzMcre +/+ and Cas9 +/- phenotype?

04 February 2022 0 8K Report

Would my Bone Marrow Derived Macrophage (BMDM) culture have Cas9 expression, even though they are heterozygote for the Cas9 gene?

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Science method

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Can some indicate a technique - or approach - to measure the thickness of a 20µm film and thickness with water uptake?

Hi, I need to measure the real-time (or temporal intervals) thickness variation of a transparent material from dry to wet/humid conditions, however, I cannot use the following techniques: -...

08 July 2024 4,799 4 View

THP-1 cells completely dying with PMA, help?

Have been trying to induce THP-1 using PMA at different concentrations (range from 5 to 50 ng/mL) but after 48h all the conditions except the control are mostly dead, checked by trypan blue and...

03 July 2024 3,884 5 View

Is there any situation where the use of negative q exponents in the formula of Hill numbers is justifiable?

Hill numbers have become an elegant and powerful unifying tool in the measurement of many levels of biological diversity. Although mathematically possible, the use of negative values for the...

09 June 2024 9,302 2 View

Is there any news on the treatment of macular injury from high-intensity LED light?

Hi everyone, I'm looking for papers regarding macular injury from LED light, but it seems there's only publications talking about acute lesions. Thanks in advance. Cheers!

13 May 2024 7,160 0 View

Can I purify large quantities of proteins using the ÄKTA protein purification system equipped with size exclusion or ion exchange columns?

Can I purify large quantities of phycocyanin (current concentration of 30%) using the ÄKTA system equipped with size exclusion or ion exchange columns? Could I perform multiple injections to...

26 March 2024 5,031 2 View

What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...?

Dear, What is the most suitable conductivity cell (k=0.1; K=1) for evaluating the conductivity of pharmaceutical formulations such as liposomes, LNPs...

24 March 2024 5,466 2 View

Barlat 1991 model. How are the parametes calculated?

I am doing a comparison between different anisotropy models. Now, I am understanding the Barlat 1991 model. I understand the concept but I have some doubts about how to proceed to calculate the...

10 March 2024 3,354 1 View

What are the best websites to find academic events?

I'm from Brazil, but I want to start proposing communications and papers for international events. Are there any specific websites I can use to find events? I really appreciate any help you can...

06 February 2024 2,489 1 View

Can Heavy metal pollution cause deffects in CaCO3 crystals from marine calcifiers?

My question is because we know that divalent ions can substitute calcium in the biomineralization process of some marine calcifiers and if we have a huge amount of free Fe2+ from pollution, for...

24 September 2023 2,697 1 View

Any suggestions for crab identification guides in sri lanka (estuarine/mangrove crabs)?

I am looking for some pictorial guides related to crab species in sri lanka. Any suggestions for such identification guides?

21 July 2023 1,800 3 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View