Can I purify large quantities of phycocyanin (current concentration of 30%) using the ÄKTA system equipped with size exclusion or ion exchange columns?
Could I perform multiple injections to isolate 100 mg of phycocyanin?
Yes, the ÄKTA protein purification system equipped with size exclusion or ion exchange columns is capable of purifying large quantities of proteins. These systems are designed to handle a wide range of sample volumes and can be scaled up to accommodate large quantities of protein.
Size exclusion chromatography (SEC) is ideal for separating proteins based on their size and molecular weight, while ion exchange chromatography (IEX) separates proteins based on their charge characteristics. Both techniques can be highly effective for purifying proteins at a large scale.
The ÄKTA system offers precise control over purification parameters such as flow rate, gradient formation, and fraction collection, ensuring efficient and reproducible purification of proteins.
However, the scale of purification achievable with ÄKTA systems may vary depending on the specific model and configuration. It's important to verify that your ÄKTA system is equipped with columns and accessories suitable for large-scale purification and to optimize your purification protocol accordingly.
Overall, the ÄKTA protein purification system is a powerful tool for purifying large quantities of proteins using size exclusion or ion exchange chromatography techniques. Rodrigo Conceicao
Yes, you can use AKTA for this purpose. However, the key considerations are your columns and the volume of available resin. For large-scale production, you will need larger columns and faster flow rates. There are several types of AKTA systems available, tailored to different scales of purification.
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