Hi,
I'm doing work on ssDNAs in solutions that are of a pH of about 2.5. The ssDNA is in this kind of solution for ~1 hour.
It was recently brought to my attention that protonation of DNA would be occurring at this pH and that acid catalyzed hydrolysis (depurination to be specific) could be braking down my oligos.
The literature seems to support this, but I haven't been able to find at what rates this occurs at. There is one paper that mentions that the rate of this depurination is dependent on the temperature and pH of the solution but it didn't really go into what the actual rate of the reaction was in degrading ssDNA. Does anyone know more about this? Should I be concerned about depurination occurring in the hour that my ssDNA is exposed to an acidic environment?
As always thank you for your thoughts and I look forward to hearing from you!
Best,
-V
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