Juliano Machado

7 Questions 8 Answers 0 Followers

Questions related from Juliano Machado

What is the correct manner to represent the effects of a treatment in autophagic flux assay in vitro?

Dear all, In an in vitro experiment of autophagic flux assay by western blot using chloroquine, is it correct to use the LC3-II/LC3-I ratio as a manner to represent the effects of a treatment or...

08 August 2018 5,352 0 View

Does anyone know if 25 or 50 uM of chloroquine treatment could be toxic for the cells (C2C12 cells or any other cell line) in a time-course treatment?

Does anyone know if 25 or 50 uM of chloroquine treatment could be toxic for the cells (C2C12 cells or any other cell line) in a time-course treatment like 1, 3, 24, and 48 h?

06 June 2018 212 1 View

Should I add 0,1% BSA to the serum-free media to produce the C26-conditioned media or just culture the cells in the serum-free media is sufficient?

Hi everyone, I want to produce conditioned media from C26 cells for then treat C2C12 myotubes with this conditioned media. I have read some papers using serum-free media for 24h to produce C26...

05 May 2018 7,738 0 View

Should I add 0,1% BSA to the serum-free media to produce the C26-conditioned media or just culture the cells in the serum-free media is sufficient?

Hi everyone, I want to produce conditioned media from C26 cells for then treat C2C12 myotubes with this conditioned media. I have read some papers using serum-free media for 24h to produce C26...

05 May 2018 1,662 0 View

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI?

Is transfection efficiency of C2C12 cells with Lipofectamine 2000 or 3000 better than PEI? And how about Fugene?

07 July 2015 8,707 7 View

What is the correct manner to represent the effects of a treatment in autophagic flux assay in vitro?

Dear all, In an in vitro experiment of autophagic flux assay by western blot using chloroquine, is it correct to use the LC3-II/LC3-I ratio as a manner to represent the effects of a treatment or...

01 January 1970 1,040 3 View

How would you all interpret the following results of autophagic flux?

Dear all, How would you all interpret the following results? Please note the cells were incubated for 24 or 48h in conditioned medium in the presence of chloroquine during whole time-course of...

01 January 1970 6,369 4 View