I harvest my cells 6 days after transfection. By checking HBV Core DNA on the membrane I rarely see the SS band while the DS (about 3kb) band is sharp and visible enough. Could it be a time dependent assay, e.g. does an earlier harvesting result in a sharper SS signal?
Khoshalam az ashnayi ba shoma.bebakhshin mishe b imylam
ba addres zir imyl bezani?
ببخشین متولد آآلمان هستین یا واسه تحصیل رفتین؟
motvald alman hasti?ya vase tahsil rafti?
Sharmande narahat shodin azam?mikhastam khahesh konam baham bishtar ashna shim
Chera javab namidi?az chi mitarsi?onam kharj ya az almanya yad gefti k mohabat dosti bade.bayad sard khoshk bod.bikhyal.har jor rahati
An earlier harvesting result does non in a sharper ss signal and ds band is sharp
It seems that it's difficult to observe ssDNA band in authentic infection. Gripon et al (PNAS 2002) showed nice rc, dsl and ccc but not ssDNA until day 4 p.i. I didn't find the ss in infected HepG2-NTCP cells yet.
However, likewise intracellular ssDNA is quite visible in transfected HepG2 cells (Figure 3, Infection Process of the Hepatitis B Virus Depends on the Presence of a Defined Sequence in the Pre-S1 Domain, J. LE SEYEC, P. CHOUTEAU, I. CANNIE, C. GUGUEN-GUILLOUZO, AND P. GRIPON, JVI, 1999).
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