Elham Shirvani Dastgerdi

5 Questions 5 Answers 0 Followers

Questions related from Elham Shirvani Dastgerdi

Can someone recommend the process needed for microRNA extraction and sequencing?

I have a fraction on my RNA blot less than100nt which I guess to be microRNA. I have no idea which kits are suitable for small RNA extraction. I got this RNA along with my main viral RNA using...

18 December 2013 7,996 7 View

Should HBV always appear with a pattern of 3 DNA bands (SS, DS and RC) in Southern blot?

I harvest my cells 6 days after transfection. By checking HBV Core DNA on the membrane I rarely see the SS band while the DS (about 3kb) band is sharp and visible enough. Could it be a time...

14 September 2013 1,308 10 View

Which cell types are normally used for HIV-1 transient transfection and which condition is requiered for the virus in these cells?

I am looking for some data about the cell types which support HIV-1 replication (a complete genome) in a transient transfection assay.

04 September 2013 5,590 5 View

Can someone recommend a software similar to Gene Runner, with such options as DNA translation in all frames, searching oligos, designing primers?

I've got a windows 7 on my PC and can't install Gene Runner on it. I searched a lot but couldn't find a software which contains all the possibilities together. Does anyone have any suggestion?

22 July 2013 484 23 View

Can plasmids pass throught the sucrose cushion along with viral particles?

I use vectors containing whole genome of HBV for my transfections. To check viral replication, I get the supernatant of the cultured hepatocytes and concentrate the virus by centrifugation over...

14 January 2013 1,134 3 View