Can anyone direct me to an existing Validated Health Care Survey/Instrument?

Hello, I am working on a paper and looking for an existing Validated Health Care Survey/Instrument. I have been performing virtual ergonomic evaluations since the start of COVID and want to...

04 February 2021 4,756 2 View

Flourescent in-situ Hybridization: How to improve Flourescein probe signals?

During FISH, we have consistently had issues getting signals FITC (flourescein) probes while Cy3 and Cy5 signals have been much easier. The weak or non-existent FITC signal is observed independent...

04 February 2020 2,966 1 View

Can any stains be used on spheroids to help visualize them for embedding, but NOT interfere with subsequent immunofluorescence?

My colleagues and I are trying to embed very small tumor spheroids and the major limitation has been actually seeing them to transfer them from culture to Histogel. We have read several reports...

24 September 2019 8,910 1 View

How do you induce murine intestinal enterospheres to develop into true organoids?

We extracted murine intestinal epithelial organoids using the StemCell Technologies protocol. We had a good number of organoids develop. I then passaged these organoids, and apparently lost them...

05 September 2019 6,950 1 View

Has anyone noticed different sizes of MSCs in culture?

My lab is currently culturing human bone-marrow mesenchymal stem cells. I've noticed when I trypsin the cells for passaging/counting that there appears to be two sizes of cell present (see image...

06 August 2019 6,121 5 View

Why do multiple T-tests say data are not significant and ANOVA says the same data is significant?

Hello, I have a data set of morphological measurements of the same character from 4 different species (all different sample sizes). Just looking at the data in boxplot format, I can see that all...

12 March 2019 2,798 6 View

How do I overcome sample matrix effect for serum without dilution (for use in ELISA)?

Hi all, I am currently optimizing an ELISA to detect myeloperoxidase in human serum. I currently use 1% BSA/PBS to block and as a diluent for the standards/samples and antibodies. My wash buffer...

24 September 2018 4,738 6 View

Is Hate or Love the dominant emotion?

Though this can be perceived to be philosophical in retrospect, it is a valid behavioral question. I contend that hate is more dominant. In observational research, not sanctioned, just in the...

07 May 2018 7,951 4 View

Sharing neutrophil isolation and RNA extraction protocol/work-flow?

I'm interested in isolating neutrophils from human whole blood for subsequent RNA extraction for downstream purposes like qPCR. Just wondering if anyone is willing to share their protocol/work...

01 February 2018 9,302 2 View

Can residual centered interactions be used in general linear models?

I have created an interaction that is residual centered. Can I use this interaction in an ANOVA, or should these only be used in regression? Many thanks.

15 January 2018 7,343 1 View

How to find the mean, SD and CI in SPSS?

Hi, I hope that someone can help me with this task: I have 5 groups of participants which are numbered in SPSS from 1 to 5 (according to their group number) and are all under the variable group....

03 March 2021 506 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Can you share me how teacher education is managed in your respective national educational system?

I feel that the practice in teacher education in my country is below the expected performance level due to very poor management system. Hope I will learn something from your experiences.

02 March 2021 1,516 4 View

CGA 300 Regulator for CO2 cylinder?

Has anyone ever tried to deliver CO2 with a CGA 300 regulator? I know that a CGA 320 regulator is standard for CO2. The issue is that I currently do not have any free CGA 320 regulators. I also...

25 February 2021 3,292 3 View

What are the patterns of human behavior that lead to COVID-19 infections?

Does data from contact tracing help in establishing patterns of behavior and social interactions that lead to infections? There are cases here in the Philippines where patients have no travel...

25 February 2021 9,002 13 View

What is a good reagent for lentiviral plasmid transfection in HeLa cells?

I have been using Lipofectamine 2000 but was hoping there may be a better protocol or one for something like Lipofectamine PLUS reagent.

24 February 2021 1,633 4 View

Can cells be reused after using WST 8/ CCK 8 from Sigma Aldrich?

Hi all, Can cells be reused after I have seed them onto 96 well plates and test them with cell counting kit at OD450 (CCK8) at 12 hours, wash them with PBS x3 and add new media and let them...

24 February 2021 5,072 1 View

Why cannot world and community leaders be gentle? Where did ethics of care go?

The whole world is in a mess and paused. We all are tired of seeing hypermasculinized and destructive leaderships all around us. This world has seen enough of the aggressive patriarchal social...

23 February 2021 292 7 View

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22 February 2021 9,932 11 View

Which type of loading is to be used in dynamic analysis of Machien foundation?

Hello Everyone, Hope you all are doing well. I'm trying to simulate a machine foundation in ABAQUS with a geo-cell reinforced soil bed. Im totally confused to which type of loading would be...

21 February 2021 4,388 3 View