02 February 2024 1 6K Report

Is there any available literature that describes, in depth, rubella antiserum titers that successfully block rubella when measuring measles potency via the TCID50 assay in a combination MR vaccine?

More Kathryn Secrist's questions See All
Can anyone direct me to an existing Validated Health Care Survey/Instrument?

Hello, I am working on a paper and looking for an existing Validated Health Care Survey/Instrument. I have been performing virtual ergonomic evaluations since the start of COVID and want to...

04 February 2021 4,756 2 View

Flourescent in-situ Hybridization: How to improve Flourescein probe signals?

During FISH, we have consistently had issues getting signals FITC (flourescein) probes while Cy3 and Cy5 signals have been much easier. The weak or non-existent FITC signal is observed independent...

04 February 2020 2,966 1 View

Can any stains be used on spheroids to help visualize them for embedding, but NOT interfere with subsequent immunofluorescence?

My colleagues and I are trying to embed very small tumor spheroids and the major limitation has been actually seeing them to transfer them from culture to Histogel. We have read several reports...

24 September 2019 8,910 1 View

How do you induce murine intestinal enterospheres to develop into true organoids?

We extracted murine intestinal epithelial organoids using the StemCell Technologies protocol. We had a good number of organoids develop. I then passaged these organoids, and apparently lost them...

05 September 2019 6,950 1 View

Has anyone noticed different sizes of MSCs in culture?

My lab is currently culturing human bone-marrow mesenchymal stem cells. I've noticed when I trypsin the cells for passaging/counting that there appears to be two sizes of cell present (see image...

06 August 2019 6,121 5 View

Why do multiple T-tests say data are not significant and ANOVA says the same data is significant?

Hello, I have a data set of morphological measurements of the same character from 4 different species (all different sample sizes). Just looking at the data in boxplot format, I can see that all...

12 March 2019 2,798 6 View

How do I overcome sample matrix effect for serum without dilution (for use in ELISA)?

Hi all, I am currently optimizing an ELISA to detect myeloperoxidase in human serum. I currently use 1% BSA/PBS to block and as a diluent for the standards/samples and antibodies. My wash buffer...

24 September 2018 4,738 6 View

Is Hate or Love the dominant emotion?

Though this can be perceived to be philosophical in retrospect, it is a valid behavioral question. I contend that hate is more dominant. In observational research, not sanctioned, just in the...

07 May 2018 7,951 4 View

Sharing neutrophil isolation and RNA extraction protocol/work-flow?

I'm interested in isolating neutrophils from human whole blood for subsequent RNA extraction for downstream purposes like qPCR. Just wondering if anyone is willing to share their protocol/work...

01 February 2018 9,302 2 View

Can residual centered interactions be used in general linear models?

I have created an interaction that is residual centered. Can I use this interaction in an ANOVA, or should these only be used in regression? Many thanks.

15 January 2018 7,343 1 View

Similar questions and discussions
SDS page software for analysis?

I did sds page to determine the difference in protein expression between 3 types of vaccine , but i don't have scanner or densitometer available. . so i wonder if there is a software to analyze...

02 March 2021 6,270 3 View

Which of the Covid-19 Vaccines would elicit the best immunoprotection and why?

I want to know which of the available Covid-19 Vaccines would elicit the best immunological response and why

02 March 2021 1,388 3 View

Is the sinovac vaccine safe and effective for people over the age of 60 ?

Dear respective researchers, I was asked by my institution to be vaccinated this week using the sinovac vaccine. However, from some of the information I got, this vaccine is not recommended for...

01 March 2021 7,781 6 View

How vaccines of covid 19 work?

Millions ask how vaccines of covid 19 be successful and safe, its here to explain generally how vaccine of pfizer work ,mRNA vaccines teach our cells how to make a protein that will trigger an...

28 February 2021 2,723 4 View

What is the most common number of epitopes in an multi-epitope Vaccine?

hi everyone, I'm interested in the field of vaccine design specifically multi-epitope cancer vaccines , I want to know if there is a minimum and maximum number of epitopes we could use in a...

27 February 2021 3,882 3 View

If a given country passes mandatory 'vaccine passport rule' to go outdoors, otherwise stay at home, can it help to vaccinate all against COVID-19?

Wether vaccine passport declared not to go out of home without it may help taking COVID-19 vaccine. Can't it break individual's right to protect the community? Your comments on perspectives...

27 February 2021 5,549 23 View

How long do vaccines protect us against COVID 19?

Or will we have to get vaccinated every year, as in the case of influenza?

27 February 2021 1,048 5 View

Any Differences in COVID-19 vaccines?

Are there any differences (outside of the clinical trials that led to their EUA) observed in the rate of infection among those who have received the Pfizer vs Moderna vaccine since the mass...

22 February 2021 9,727 6 View

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

Can COVID-19 vaccines protect against the emerging strains of SARS-CoV-2?

18 February 2021 8,221 8 View

Would different strains of SARS-CoV-2 required different vaccines shots?

Would different strains of SARS-CoV-2 required different vaccines shots?

18 February 2021 6,934 6 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close