Kathryn Hally

2 Questions 3 Answers 0 Followers

Questions related from Kathryn Hally

How do I overcome sample matrix effect for serum without dilution (for use in ELISA)?

Hi all, I am currently optimizing an ELISA to detect myeloperoxidase in human serum. I currently use 1% BSA/PBS to block and as a diluent for the standards/samples and antibodies. My wash buffer...

25 September 2018 4,809 6 View

Sharing neutrophil isolation and RNA extraction protocol/work-flow?

I'm interested in isolating neutrophils from human whole blood for subsequent RNA extraction for downstream purposes like qPCR. Just wondering if anyone is willing to share their protocol/work...

02 February 2018 9,394 2 View