We have put into a continuous culturing of 15days NIT1 (B.cells) cell line in a DMEM basal medium and we have obtained a few colonies as illustrated in the attached images.
From my experience, it is not good to keep the cells in the same container for a long time. If the cells did not grow, this means that you did not choose the suitable density of cells compared to the size of the tissue culture container. Cells need to support each other in order to grow. I advise you to trypsinize the cells and move them to a smaller tissue culture flask. Hope this helps
Hello Feriel Sellam
What is the purpose of the study? If you are just maintaining the cells in culture, you need to supplement the basal medium with 10% FBS. The doubling time is around 46-50 hours. The cells may be growing slowly for want of nutrients and may eventually die. The basal medium will not contain growth factors, hormones, binding proteins, and other factors that are necessary for the growth and survival of cells. So, you need to passage the cells in a new culture flask in DMEM containing 10% FBS.
Best.
Many thanks for your answer, we have already supplemented our medium with 15% FBS, we have just forggotten to state it. Please, if you have any futher suggestions we would be thankful to receive them.
Best
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