Dear Christoph!

Please You look at these SF126 cell culture protocols:

Article Inhibiting receptor tyrosine kinase AXL with small molecule ...

Cell culture

Human high-grade glioma cells U118MG were obtained from American Type Culture Collection (ATCC). Human high-grade glioma cells SF126 were obtained from the JCRB Cell Bank. Both were primary tumor cell cultures derived from surgical specimens of human GBM, WHO Grade IV. Tumor cells were maintained as monolayer cultured in tumor growth medium at 37°C, 5% CO2, 95% humidity in a tissue culture incubator. Growth medium was comprised of Dulbecco's modified Eagle's medium (DMEM, Invitrogen) supplemented with 10% fetal calf serum (FCS) and 1% antibiotics (penicillin/steptamycin). Prior starting experiments, each cell population was grown in equal (80%) confluence in culture dishes under normal conditions or starving conditions (culture medium containing 0% FCS).

https://academic.oup.com/carcin/article/35/4/877/269357

Glioma cell lines

The glioma cell lines U251 and U87MG were originally obtained from the American Type Culture Collection (ATCC). The SF126, SF188 and SF539 cell lines were originally from the Brain Tumor Research Center of San Francisco. It should be noted that according to the ATCC and upon sequencing, the authenticity of U-251 cells has been questioned and this cell line has been suggested to be similar to U-373MG cells. However, since U-373MG cells are also glioblastomas, the results of this work are not affected in any way by the controversy. The GM2493, GM133 and GM1600 cell lines were a kind gift from Dr Paul S.Mischel [School of Medicine at UCLA (University of California, Los Angeles), Los Angeles, CA]. All cells were maintained in Dulbecco’s modified Eagle’s medium with L-glutamine (Life Technologies, Invitrogen Corp.), 10% fetal bovine serum (Sigma–Aldrich) and 1% penicillin–streptomycin (Life Technologies, Invitrogen) and grown at 37°C

Dear Alexandr,

thanks for the suggestion, however in this paper the start with 5000 cells/well, we want to start with 1 cell/well.